Protein transfer from isoelectric focusing gels: The native blot

Michael P. Reinhart; Daniel Malamud

doi:10.1016/0003-2697(82)90439-0

Protein transfer from isoelectric focusing gels: The native blot

Michael P. Reinhart, Daniel Malamud

Research output: Contribution to journal › Article › peer-review

Abstract

The nonelectrophoretic transfer of proteins from thin (0.5 mm) isoelectric focusing gels to nitrocellulose was complete in 1 h. Blotting was bidirectional with 60% of the protein transferred to the top blot and the remaining 40% to the bottom blot. The use of nondenaturing transfer buffers permits proteins to be blotted in the native state. Immunological determinants are preserved and enzyme activity can be detected on the blots.

Original language	English (US)
Pages (from-to)	229-235
Number of pages	7
Journal	Analytical Biochemistry
Volume	123
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(82)90439-0
State	Published - Jul 1 1982

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0003-2697(82)90439-0

Cite this

@article{a00baada95b8451f992126c3f0c9aebd,

title = "Protein transfer from isoelectric focusing gels: The native blot",

abstract = "The nonelectrophoretic transfer of proteins from thin (0.5 mm) isoelectric focusing gels to nitrocellulose was complete in 1 h. Blotting was bidirectional with 60% of the protein transferred to the top blot and the remaining 40% to the bottom blot. The use of nondenaturing transfer buffers permits proteins to be blotted in the native state. Immunological determinants are preserved and enzyme activity can be detected on the blots.",

author = "Reinhart, {Michael P.} and Daniel Malamud",

note = "Funding Information: We would like to thank Ms. Cheryl Davis and Mr. Mark Desrosiers for their expert technical assistance. This research was supported by NIH Grants DE-05462, CA-28395, and CA-06979.",

year = "1982",

month = jul,

day = "1",

doi = "10.1016/0003-2697(82)90439-0",

language = "English (US)",

volume = "123",

pages = "229--235",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Protein transfer from isoelectric focusing gels

T2 - The native blot

AU - Reinhart, Michael P.

AU - Malamud, Daniel

N1 - Funding Information: We would like to thank Ms. Cheryl Davis and Mr. Mark Desrosiers for their expert technical assistance. This research was supported by NIH Grants DE-05462, CA-28395, and CA-06979.

PY - 1982/7/1

Y1 - 1982/7/1

N2 - The nonelectrophoretic transfer of proteins from thin (0.5 mm) isoelectric focusing gels to nitrocellulose was complete in 1 h. Blotting was bidirectional with 60% of the protein transferred to the top blot and the remaining 40% to the bottom blot. The use of nondenaturing transfer buffers permits proteins to be blotted in the native state. Immunological determinants are preserved and enzyme activity can be detected on the blots.

AB - The nonelectrophoretic transfer of proteins from thin (0.5 mm) isoelectric focusing gels to nitrocellulose was complete in 1 h. Blotting was bidirectional with 60% of the protein transferred to the top blot and the remaining 40% to the bottom blot. The use of nondenaturing transfer buffers permits proteins to be blotted in the native state. Immunological determinants are preserved and enzyme activity can be detected on the blots.

UR - http://www.scopus.com/inward/record.url?scp=0020163542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020163542&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(82)90439-0

DO - 10.1016/0003-2697(82)90439-0

M3 - Article

C2 - 6181706

AN - SCOPUS:0020163542

SN - 0003-2697

VL - 123

SP - 229

EP - 235

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Protein transfer from isoelectric focusing gels: The native blot

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this