Proteomic Tools to Study the Effect of BDNF on De Novo Protein Synthesis

Heather Bowling, Eric Klann

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

BDNF has been long recognized as a potent contributor to neural development, maintenance, and plasticity, and previous studies have revealed protein synthesis to be a major effector in its molecular mechanisms of action. Until recently, the only tools available to study protein synthesis were unlabeled proteomics and radioactive amino acid labeling. The emergence of new tools in the 2000s has led to more precise and in-depth understanding of the mechanism of action of BDNF at the protein synthesis level. In this chapter, we will provide an overview of some of these methods—SUnSET, BONCAT, FUNCAT, and BONLAC—for the study of BDNF-induced de novo protein synthesis.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages217-239
Number of pages23
DOIs
StatePublished - 2019

Publication series

NameNeuromethods
Volume143
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • BONCAT
  • BONLAC
  • FUNCAT
  • Neuroproteomics
  • SUnSET

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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  • Cite this

    Bowling, H., & Klann, E. (2019). Proteomic Tools to Study the Effect of BDNF on De Novo Protein Synthesis. In Neuromethods (pp. 217-239). (Neuromethods; Vol. 143). Humana Press Inc.. https://doi.org/10.1007/7657_2018_16