Protocol to generate and characterize biofouling transformants of a model marine diatom

Weiqi Fu; Bushra Dohai; Diana Charles El Assal; Sarah Daakour; Amnah Alzahmi; David R. Nelson; Ashish Jaiswal; Alexandra Mystikou; Mehar Sultana; James Weston; Kourosh Salehi-Ashtiani

doi:10.1016/j.xpro.2021.100716

Protocol to generate and characterize biofouling transformants of a model marine diatom

Weiqi Fu, Bushra Dohai, Diana Charles El Assal, Sarah Daakour, Amnah Alzahmi, David R. Nelson, Ashish Jaiswal, Alexandra Mystikou, Mehar Sultana, James Weston, Kourosh Salehi-Ashtiani

Research output: Contribution to journal › Article › peer-review

Abstract

Diatoms are a major group of microalgae that initiate biofouling by surface colonization of human-made underwater structures; however, the involved regulatory pathways remain uncharacterized. Here, we describe a protocol for identifying and validating regulatory genes involved in the morphology shift of the model diatom species Phaeodactylum tricornutum during surface colonization. We also provide a workflow for characterizing biofouling transformants. By using this protocol, gene targets such as GPCR signaling genes could be identified and manipulated to turn off diatom biofouling. For complete information on the generation and use of this protocol, please refer to Fu et al. (2020).

Original language	English (US)
Article number	100716
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100716
State	Published - Sep 17 2021

Keywords

Biotechnology and bioengineering
Microbiology
Model Organisms
RNAseq
Systems biology

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Medicine

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10.1016/j.xpro.2021.100716

Cite this

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title = "Protocol to generate and characterize biofouling transformants of a model marine diatom",

abstract = "Diatoms are a major group of microalgae that initiate biofouling by surface colonization of human-made underwater structures; however, the involved regulatory pathways remain uncharacterized. Here, we describe a protocol for identifying and validating regulatory genes involved in the morphology shift of the model diatom species Phaeodactylum tricornutum during surface colonization. We also provide a workflow for characterizing biofouling transformants. By using this protocol, gene targets such as GPCR signaling genes could be identified and manipulated to turn off diatom biofouling. For complete information on the generation and use of this protocol, please refer to Fu et al. (2020).",

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doi = "10.1016/j.xpro.2021.100716",

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AU - Fu, Weiqi

AU - Dohai, Bushra

AU - El Assal, Diana Charles

AU - Daakour, Sarah

AU - Alzahmi, Amnah

AU - Nelson, David R.

AU - Jaiswal, Ashish

AU - Mystikou, Alexandra

AU - Sultana, Mehar

AU - Weston, James

AU - Salehi-Ashtiani, Kourosh

PY - 2021/9/17

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AB - Diatoms are a major group of microalgae that initiate biofouling by surface colonization of human-made underwater structures; however, the involved regulatory pathways remain uncharacterized. Here, we describe a protocol for identifying and validating regulatory genes involved in the morphology shift of the model diatom species Phaeodactylum tricornutum during surface colonization. We also provide a workflow for characterizing biofouling transformants. By using this protocol, gene targets such as GPCR signaling genes could be identified and manipulated to turn off diatom biofouling. For complete information on the generation and use of this protocol, please refer to Fu et al. (2020).

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KW - Model Organisms

KW - RNAseq

KW - Systems biology

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Protocol to generate and characterize biofouling transformants of a model marine diatom

Abstract

Keywords

ASJC Scopus subject areas

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