Protocol to generate and characterize biofouling transformants of a model marine diatom

Weiqi Fu, Bushra Dohai, Diana Charles El Assal, Sarah Daakour, Amnah Alzahmi, David R. Nelson, Ashish Jaiswal, Alexandra Mystikou, Mehar Sultana, James Weston, Kourosh Salehi-Ashtiani

Research output: Contribution to journalArticlepeer-review


Diatoms are a major group of microalgae that initiate biofouling by surface colonization of human-made underwater structures; however, the involved regulatory pathways remain uncharacterized. Here, we describe a protocol for identifying and validating regulatory genes involved in the morphology shift of the model diatom species Phaeodactylum tricornutum during surface colonization. We also provide a workflow for characterizing biofouling transformants. By using this protocol, gene targets such as GPCR signaling genes could be identified and manipulated to turn off diatom biofouling. For complete information on the generation and use of this protocol, please refer to Fu et al. (2020).

Original languageEnglish (US)
Article number100716
JournalSTAR Protocols
Issue number3
StatePublished - Sep 17 2021


  • Biotechnology and bioengineering
  • Microbiology
  • Model Organisms
  • RNAseq
  • Systems biology

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Medicine


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