Protocols for generation of immortalized human brown and white preadipocyte cell lines

Farnaz Shamsi, Yu Hua Tseng

Research output: Chapter in Book/Report/Conference proceedingChapter


Human brown and white preadipocytes offer unique cell models to study human adipogenesis and thermogenesis. Here, we describe the detailed procedures for isolation of human brown and white predipocytes from deep and superficial neck fat. To grow these cells in vitro for a prolonged period of time, they should be immortalized following the procedure discussed here. We also provide the protocol for expansion, cryopreservation, and adipogenic differentiation of cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2017

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745


  • Brown and white adipose tissue
  • Differentiation
  • Immortalization
  • Neck fat
  • Preadipocytes
  • Stromal vascular fraction (SVF)
  • Gene Expression
  • Humans
  • Adipocytes, Brown/cytology
  • Transfection
  • Adipocytes, White/cytology
  • Cell Differentiation
  • Cell Culture Techniques
  • Cell Line, Transformed
  • Stem Cells/cytology
  • Telomerase/genetics

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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