@inbook{af33acdf0de74f64b89ad1369300f2b1,
title = "Purification of fluorescent labeled cells from dissociated Ciona embryos",
abstract = "Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.",
keywords = "Cell dissociation, Cell-lineage specific population, MACS, Mosaic expression, Multi-channel combinatorial FACS, RNA extraction, TVC",
author = "Wei Wang and Claudia Racioppi and Basile Gravez and Lionel Christiaen",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Nature Singapore Pte Ltd.",
year = "2018",
doi = "10.1007/978-981-10-7545-2_9",
language = "English (US)",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer New York LLC",
pages = "101--107",
booktitle = "Advances in Experimental Medicine and Biology",
}