Quantification of tissue amyloid content in AA amyloidosis by inhibition ELISA

Shoshana Yakar, Batia Kaplan, Galina German, Avi Livneh, Katsutoshi Miura, Shmuel Shtrasburg, Mordechai Pras

Research output: Contribution to journalArticlepeer-review

Abstract

The determination of the amount of amyloid in tissues is important to follow the onset and the development of amyloidosis. In the present study a new procedure for quantification of amyloid proteins in AA amyloidosis is described. It involves the protein extraction from mouse and human tissue and evaluation of their amount by inhibition ELISA. The procedure is simple and may be performed using milligram amounts of tissue. The sensitivity limit of protein AA detection corresponds to 0.007mg/mg tissue. The results obtained using the inhibition ELISA were consistent with those obtained by Congo red staining. In contrast to previously developed amyloid quantification techniques, the presented procedure required only the basic equipment avail-able in most laboratories.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalAmyloid
Volume2
Issue number3
DOIs
StatePublished - 1995

Keywords

  • Aa amyloidosis
  • Human and murine proteins aa
  • Inhibition elisa

ASJC Scopus subject areas

  • Internal Medicine

Fingerprint Dive into the research topics of 'Quantification of tissue amyloid content in AA amyloidosis by inhibition ELISA'. Together they form a unique fingerprint.

Cite this