TY - GEN
T1 - Quantifying resource competition and its effects in the TX-TL system
AU - Gyorgy, Andras
AU - Murray, Richard M.
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2016/12/27
Y1 - 2016/12/27
N2 - Without accounting for the limited availability of shared cellular resources, the standard model of gene expression fails to reliably predict experimental data obtained in vitro. To overcome this limitation, we develop a dynamical model of gene expression explicitly modeling competition for scarce resources. In addition to accurately describing the experimental data, this model only depends on a handful of easily identifiable parameters with clear physical interpretation. Based on this model, we then characterize the combinations of protein concentrations that are simultaneously realizable with shared resources. As application examples, we demonstrate how the results can be used to explain similarities/differences among different in vitro extracts, furthermore, we illustrate that accounting for resource usage is essential in circuit design considering the toggle switch.
AB - Without accounting for the limited availability of shared cellular resources, the standard model of gene expression fails to reliably predict experimental data obtained in vitro. To overcome this limitation, we develop a dynamical model of gene expression explicitly modeling competition for scarce resources. In addition to accurately describing the experimental data, this model only depends on a handful of easily identifiable parameters with clear physical interpretation. Based on this model, we then characterize the combinations of protein concentrations that are simultaneously realizable with shared resources. As application examples, we demonstrate how the results can be used to explain similarities/differences among different in vitro extracts, furthermore, we illustrate that accounting for resource usage is essential in circuit design considering the toggle switch.
UR - http://www.scopus.com/inward/record.url?scp=85010756895&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010756895&partnerID=8YFLogxK
U2 - 10.1109/CDC.2016.7798775
DO - 10.1109/CDC.2016.7798775
M3 - Conference contribution
AN - SCOPUS:85010756895
T3 - 2016 IEEE 55th Conference on Decision and Control, CDC 2016
SP - 3363
EP - 3368
BT - 2016 IEEE 55th Conference on Decision and Control, CDC 2016
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 55th IEEE Conference on Decision and Control, CDC 2016
Y2 - 12 December 2016 through 14 December 2016
ER -