TY - JOUR
T1 - Quantitation of catecholamines in inflamed human dental pulp by high-performance liquid chromatography
AU - Nup, Caroline
AU - Rosenberg, Paul
AU - Linke, Harald
AU - Tordik, Patricia
N1 - Funding Information:
This investigation is part of an ongoing investigation into the role of catecholamines in pulp tissue. The initial study, “Quantitation of Catecholamines in Uninflamed Human Dental Pulp Tissues by High-performance Liquid Chromatography,” was supported in part by the American Association of Endodontists (AAE) and the Dean’s Faculty Research Award at New York University College of Dentistry. This investigation was also supported by the New York University College of Dentistry Dean’s Faculty Research Fund.
PY - 2001
Y1 - 2001
N2 - Catecholamines may play an important role in the control of intrapulpal pressure as mediators of vasoconstriction. A baseline level of catecholamines (dopamine, epinephrine, and norepinephrine) in the uninflamed human dental pulp was previously reported using high-performance liquid chromatography. The purpose of this study was to compare the level of catecholamines present in the inflamed human dental pulp with the baseline level established in virgin teeth. Twelve uninflamed pulps were analyzed as a control and to validate previous findings. Pulp tissue was obtained from 10 vital and inflamed teeth requiring endodontic treatment. Selective criteria for each patient included: absence of systemic disease, medications, and allergies; a vital response to ice, heat, and electric pulp tests; and periodontal probing ≤ 3 mm. A prior history of pain associated with the tooth was an additional criterion for inflamed pulps. To avoid the presence of an exogenous catecholamine, local anesthesia without epinephrine was administered. Dopamine, epinephrine, and norepinephrine were chemically extracted and analyzed by high-performance liquid chromatography with ultraviolet detection. Catecholamine levels found to be present in the pulp during inflammation were greater than the baseline level established in uninflamed pulp tissue.
AB - Catecholamines may play an important role in the control of intrapulpal pressure as mediators of vasoconstriction. A baseline level of catecholamines (dopamine, epinephrine, and norepinephrine) in the uninflamed human dental pulp was previously reported using high-performance liquid chromatography. The purpose of this study was to compare the level of catecholamines present in the inflamed human dental pulp with the baseline level established in virgin teeth. Twelve uninflamed pulps were analyzed as a control and to validate previous findings. Pulp tissue was obtained from 10 vital and inflamed teeth requiring endodontic treatment. Selective criteria for each patient included: absence of systemic disease, medications, and allergies; a vital response to ice, heat, and electric pulp tests; and periodontal probing ≤ 3 mm. A prior history of pain associated with the tooth was an additional criterion for inflamed pulps. To avoid the presence of an exogenous catecholamine, local anesthesia without epinephrine was administered. Dopamine, epinephrine, and norepinephrine were chemically extracted and analyzed by high-performance liquid chromatography with ultraviolet detection. Catecholamine levels found to be present in the pulp during inflammation were greater than the baseline level established in uninflamed pulp tissue.
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U2 - 10.1097/00004770-200102000-00001
DO - 10.1097/00004770-200102000-00001
M3 - Article
C2 - 11491641
AN - SCOPUS:0035261632
SN - 0099-2399
VL - 27
SP - 73
EP - 75
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 2
ER -