Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy

Giovanni Sena, Zak Frentz, Kenneth D. Birnbaum, Stanislas Leibler

Research output: Contribution to journalArticlepeer-review


To understand dynamic developmental processes, living tissues have to be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image, at cellular resolution, a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, to track cellular nuclei and to identify cell divisions. We demonstrate the system's capabilities by collecting data on divisions and nuclear dynamics.

Original languageEnglish (US)
Article numbere21303
JournalPloS one
Issue number6
StatePublished - 2011

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy'. Together they form a unique fingerprint.

Cite this