Quantitative cytological feature analysis by microfluidic image cytometry reveals phenotypic differences among human pluripotent stem cell lines

Ken ichiro Kamei, Minori Ohashi, Nicholas A. Graham, Yong Chen, Amander T. Clark, Owen N. Witte, Thomas G. Graeber, April D. Pyle, Hsian Rong Tseng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Human pluripotent stem cells (hPSCs) have a great potential for the application in clinical uses and basic developmental biology. Here, we introduce the new quantitative and multiparameteric methods based on microfluidic image cytometry to characterize individual hPSC behavior under a collection of self-renewing and differentiating conditions. In conjunction with microfluidic image cytometry and biostatistical analysis, we are able to evaluate and summarize the phenotypic differences among the hPSC lines.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages148-150
Number of pages3
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Human pluripotent stem cells
  • Image cytometry
  • Microfluidics
  • Single cell profiling;

ASJC Scopus subject areas

  • Control and Systems Engineering

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