@article{aed8bd1d63f6428d96f12bf797e64733,
title = "Quantitative Mapping of a Digenic Behavioral Trait Implicates Globin Variation in C. elegans Sensory Behaviors",
abstract = "Most heritable behavioral traits have a complex genetic basis, but few multigenic traits are understood at a molecular level. Here we show that the C. elegans strains N2 and CB4856 have opposite behavioral responses to simultaneous changes in environmental O2 and CO2. We identify two quantitative trait loci (QTL) that affect this trait and map each QTL to a single-gene polymorphism. One gene, npr-1, encodes a previously described neuropeptide receptor whose high activity in N2 promotes CO2 avoidance. The second gene, glb-5, encodes a neuronal globin domain protein whose high activity in CB4856 modifies behavioral responses to O2 and combined O2/CO2 stimuli. glb-5 acts in O2-sensing neurons to increase O2-evoked calcium signals, implicating globins in sensory signaling. An analysis of wild C. elegans strains indicates that the N2 alleles of npr-1 and glb-5 arose recently in the same strain background, possibly as an adaptation to laboratory conditions.",
keywords = "DEVBIO, MOLNEURO, SIGNALING",
author = "McGrath, {Patrick T.} and Rockman, {Matthew V.} and Manuel Zimmer and Heeun Jang and Macosko, {Evan Z.} and Leonid Kruglyak and Bargmann, {Cornelia I.}",
note = "Funding Information: We thank members of the Bargmann laboratory for critical help, advice, and comments on the manuscript; Phil Anderson, Jonathan Hodgkin, Carl Johnson, Nancy Lu, Warwick Nicholas, Patrick Phillips, and Sydney Brenner for sharing information about the historical isolation of C. elegans strains; and Marie-Anne Felix, Antoine Barriere, Michael Ailion, Jody Hey, and Elie Dolgin for wild-caught strains. This work was supported by the Howard Hughes Medical Institute (C.I.B.), the NIH (R01 HG004321 to L.K. and P50 GM071508 to the Lewis-Sigler Institute), the James S. McDonnell Foundation Centennial Fellowship (L.K.), the Damon Runyon Cancer Research Foundation (P.T.M.), and the Jane Coffin Childs Foundation (M.V.R.). C.I.B. and L.K. are Investigators of the Howard Hughes Medical Institute. P.T.M. designed and performed experiments, analyzed data, and wrote the paper; M.V.R designed and performed experiments and analyzed data; M.Z. and H.J. performed experiments; E.Z.M. created reagents; L.K. designed experiments and analyzed data; and C.I.B. designed experiments, analyzed data, and wrote the paper. ",
year = "2009",
month = mar,
day = "12",
doi = "10.1016/j.neuron.2009.02.012",
language = "English (US)",
volume = "61",
pages = "692--699",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "5",
}