TY - JOUR
T1 - Quantitative RT-PCR analyses of five evolutionary conserved genes in Alligator brains during development
AU - Wilson, Sarah M.
AU - Zhu, Tianli
AU - Khanna, Rajesh
AU - Pritz, Michael B.
N1 - Funding Information:
Partial support for this publication was provided by a Project Development Team grant within the ICTSI NIH/NCRR Grant Number RR025761 (to R.K.), by grants from the Ralph W. and Grace M. Showalter foundation (to R.K.), and by the Elwert Award in Medicine (to R.K.). S.M.W. is a Paul and Carole Stark Scholar. R.M. Elsey and the Rockefeller Wildlife Refuge generously provided Alligator eggs. M. Yurk aided in manuscript preparation. B. D. Pritz helped in the preparation of Figure 1.
PY - 2011/12
Y1 - 2011/12
N2 - Gene expression was investigated in the major brain subdivisions (telencephalon, diencephalon, midbrain and hindbrain) in a representative reptile, Alligator mississipiensis, during the later stages of embryonic development. The following genes were examined: voltage-gated sodium channel isoforms: NaV1.1 and NaV1.2; synaptic vesicle 2a (SV2a); synaptophysin; and calbindin 2. With the exception of synaptophysin, which was only expressed in the telencephalon, all genes were expressed in all brain regions sampled at the time periods examined. For NaV1.1, gene expression varied according to brain area sampled. When compared with NaV1.1, the pattern of NaV1.2 gene expression differed appreciably. The gene expression of SV2a was the most robust of any of the genes examined. Of the other genes examined, although differences were noted, no statistically significant changes were found either between brain part or time interval. Although limited, the present analysis is the first quantitative mRNA gene expression study in any reptile during development. Together with future experiments of a similar nature, the present gene expression results should determine which genes are expressed in major brain areas at which times during development in Alligator. When compared with other amniotes, these results will prove useful for determining how gene expression during development influences adult brain structure.
AB - Gene expression was investigated in the major brain subdivisions (telencephalon, diencephalon, midbrain and hindbrain) in a representative reptile, Alligator mississipiensis, during the later stages of embryonic development. The following genes were examined: voltage-gated sodium channel isoforms: NaV1.1 and NaV1.2; synaptic vesicle 2a (SV2a); synaptophysin; and calbindin 2. With the exception of synaptophysin, which was only expressed in the telencephalon, all genes were expressed in all brain regions sampled at the time periods examined. For NaV1.1, gene expression varied according to brain area sampled. When compared with NaV1.1, the pattern of NaV1.2 gene expression differed appreciably. The gene expression of SV2a was the most robust of any of the genes examined. Of the other genes examined, although differences were noted, no statistically significant changes were found either between brain part or time interval. Although limited, the present analysis is the first quantitative mRNA gene expression study in any reptile during development. Together with future experiments of a similar nature, the present gene expression results should determine which genes are expressed in major brain areas at which times during development in Alligator. When compared with other amniotes, these results will prove useful for determining how gene expression during development influences adult brain structure.
KW - Alligator
KW - Calbindin 2
KW - Synaptic vesicle protein 2
KW - Synaptophysin
KW - Voltage-gated sodium channel
KW - qPCR
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U2 - 10.2478/s13380-011-0036-z
DO - 10.2478/s13380-011-0036-z
M3 - Article
AN - SCOPUS:84856242747
SN - 2081-3856
VL - 2
SP - 339
EP - 343
JO - Translational Neuroscience
JF - Translational Neuroscience
IS - 4
ER -