Rapid and spatially seperated hetereogenous 3d cellular patterning using electrohydrodynamics

Anoop Menachery, Abhishek Vembadi, Pavithra Sukumar, Rachid Rezgui, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an electrically actuated approach for creating heterogeneous 3D micro-tissues using HeLa cancer cells and human foreskin fibroblasts models. In this methodology, we sequentially assemble cell types to ensure distinct spatial positioning for forming a dense heterogeneous cluster. An optimized sinusoidal voltage of 50 kHz was applied to the interdigitated electrode chip for inducing fluid flow to carry cells and deposit them in the center of the sessile droplet. This method is rapid, robust, and can provide repeatable cell micro-tissues using various cell concentrations and cell types.

Original languageEnglish (US)
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages334-335
Number of pages2
ISBN (Electronic)9781733419000
StatePublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: Oct 27 2019Oct 31 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period10/27/1910/31/19

Keywords

  • 3D cell co-culture
  • Dielectrophoresis
  • Electrokinetics
  • Sessile droplet
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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