Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB)

Paul L.A.M. Corstjens; Zongyuang Chen; Michel Zuiderwijk; Haim H. Bau; William R. Abrams; Daniel Malamud; R. Sam Niedbala; Hans J. Tanke

doi:10.1196/annals.1384.016

Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB)

Paul L.A.M. Corstjens, Zongyuang Chen, Michel Zuiderwijk, Haim H. Bau, William R. Abrams, Daniel Malamud, R. Sam Niedbala, Hans J. Tanke

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A novel assay is described formultiplex detection of antibodies against different pathogens from a single sample. The assay employs a modified lateral flow format (consecutive flow, CF) together with a sensitive reporter particle technology (up-converting phosphor technology, UPT) that allows for fully instrumented assay analysis. Lateral flow (LF) strips developed for the detection of human antibodies against human immunodeficiency virus type-1 and -2 (HIV-1 and -2) with additional capture zones to detect antibodies against Myobacterium tuberculosis (TB) and hepatitis C Virus (HCV) provided the strips to test multiplexing. Data are presented that show the performance of the TB and HCV test, as well as two multiplex assays, TB with HIV and HCV with HIV. The TB/HCV assays demonstrate excellent detection capability, and HIV multiplexing does not affect the qualitative test result. The bench-top CF format was converted to a microfluidic platformand a first prototype semiautomated chip capable of performing CF is presented here.

Original language	English (US)
Title of host publication	Oral-based Diagnostics
Publisher	Blackwell Publishing Inc.
Pages	437-445
Number of pages	9
ISBN (Print)	157331661X, 9781573316613
DOIs	https://doi.org/10.1196/annals.1384.016
State	Published - Mar 2007

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1098
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

HCV
HIV
Infectious disease
Lateral flow
Multiplex assay
TB
Up-converting phosphor

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
History and Philosophy of Science

Access to Document

10.1196/annals.1384.016

Cite this

Corstjens, P. L. A. M., Chen, Z., Zuiderwijk, M., Bau, H. H., Abrams, W. R., Malamud, D., Niedbala, R. S., & Tanke, H. J. (2007). Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB). In Oral-based Diagnostics (pp. 437-445). (Annals of the New York Academy of Sciences; Vol. 1098). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1384.016

Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB). / Corstjens, Paul L.A.M.; Chen, Zongyuang; Zuiderwijk, Michel et al.
Oral-based Diagnostics. Blackwell Publishing Inc., 2007. p. 437-445 (Annals of the New York Academy of Sciences; Vol. 1098).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Corstjens, PLAM, Chen, Z, Zuiderwijk, M, Bau, HH, Abrams, WR, Malamud, D, Niedbala, RS & Tanke, HJ 2007, Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB). in Oral-based Diagnostics. Annals of the New York Academy of Sciences, vol. 1098, Blackwell Publishing Inc., pp. 437-445. https://doi.org/10.1196/annals.1384.016

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AB - A novel assay is described formultiplex detection of antibodies against different pathogens from a single sample. The assay employs a modified lateral flow format (consecutive flow, CF) together with a sensitive reporter particle technology (up-converting phosphor technology, UPT) that allows for fully instrumented assay analysis. Lateral flow (LF) strips developed for the detection of human antibodies against human immunodeficiency virus type-1 and -2 (HIV-1 and -2) with additional capture zones to detect antibodies against Myobacterium tuberculosis (TB) and hepatitis C Virus (HCV) provided the strips to test multiplexing. Data are presented that show the performance of the TB and HCV test, as well as two multiplex assays, TB with HIV and HCV with HIV. The TB/HCV assays demonstrate excellent detection capability, and HIV multiplexing does not affect the qualitative test result. The bench-top CF format was converted to a microfluidic platformand a first prototype semiautomated chip capable of performing CF is presented here.

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Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB)

Abstract

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Keywords

ASJC Scopus subject areas

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