Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB)

Paul L.A.M. Corstjens, Zongyuang Chen, Michel Zuiderwijk, Haim H. Bau, William R. Abrams, Daniel Malamud, R. Sam Niedbala, Hans J. Tanke

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel assay is described formultiplex detection of antibodies against different pathogens from a single sample. The assay employs a modified lateral flow format (consecutive flow, CF) together with a sensitive reporter particle technology (up-converting phosphor technology, UPT) that allows for fully instrumented assay analysis. Lateral flow (LF) strips developed for the detection of human antibodies against human immunodeficiency virus type-1 and -2 (HIV-1 and -2) with additional capture zones to detect antibodies against Myobacterium tuberculosis (TB) and hepatitis C Virus (HCV) provided the strips to test multiplexing. Data are presented that show the performance of the TB and HCV test, as well as two multiplex assays, TB with HIV and HCV with HIV. The TB/HCV assays demonstrate excellent detection capability, and HIV multiplexing does not affect the qualitative test result. The bench-top CF format was converted to a microfluidic platformand a first prototype semiautomated chip capable of performing CF is presented here.

Original languageEnglish (US)
Title of host publicationOral-based Diagnostics
PublisherBlackwell Publishing Inc.
Pages437-445
Number of pages9
ISBN (Print)157331661X, 9781573316613
DOIs
StatePublished - Mar 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1098
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • HCV
  • HIV
  • Infectious disease
  • Lateral flow
  • Multiplex assay
  • TB
  • Up-converting phosphor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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