Abstract
Pyridoxal-5′-phosphate (PLP) represents an active form of Vitamin B6 that shows relatively fast imine formation with hydrazines under physiological conditions without the need of a catalyst. A convenient phosphate/amine conjugation protocol was developed to covalently link PLP to proteins, affording proteins capable of hydrazone formation with bioorthogonal hydrazinyl functional groups. Thus, the lectin Concanavalin A (Con A) was labeled with PLP. Pretreatment with fluorescein hydrazide gave dye-labeled Con A that labeled cell surfaces efficiently. Alternatively, pretargeting was achieved by labeling cells with Con A-PLP, then treatment in vitro with Alexa Fluor 488 hydrazide.
Original language | English (US) |
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Pages (from-to) | 2329-2334 |
Number of pages | 6 |
Journal | Bioconjugate Chemistry |
Volume | 23 |
Issue number | 12 |
DOIs | |
State | Published - Dec 19 2012 |
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry