Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip

Lucia S. Cheung; Xi Wei; Diogo Martins; Yong Ak Song

doi:10.1063/1.5009719

Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip

Lucia S. Cheung, Xi Wei, Diogo Martins, Yong Ak Song

Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.

Original language	English (US)
Article number	014104
Journal	Biomicrofluidics
Volume	12
Issue number	1
DOIs	https://doi.org/10.1063/1.5009719
State	Published - Jan 1 2018

ASJC Scopus subject areas

Biomedical Engineering
General Materials Science
Condensed Matter Physics
Fluid Flow and Transfer Processes
Colloid and Surface Chemistry

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10.1063/1.5009719

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title = "Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip",

abstract = "An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.",

author = "Cheung, {Lucia S.} and Xi Wei and Diogo Martins and Song, {Yong Ak}",

note = "Funding Information: We gratefully acknowledge the financial support from the New York University Abu Dhabi (NYUAD) through the NYUAD Research Enhancement Fund and Abu Dhabi Education Council Award for Research Excellence 2015. The device fabrication was conducted in the microfabrication core facility of NYUAD. We would also like to thank Professor Rastislav Levicky, Department of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, Brooklyn, New York, US, for his discussion on MO-miRNA hybridization. Publisher Copyright: {\textcopyright} 2018 Author(s).",

year = "2018",

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doi = "10.1063/1.5009719",

language = "English (US)",

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AU - Cheung, Lucia S.

AU - Wei, Xi

AU - Martins, Diogo

AU - Song, Yong Ak

N1 - Funding Information: We gratefully acknowledge the financial support from the New York University Abu Dhabi (NYUAD) through the NYUAD Research Enhancement Fund and Abu Dhabi Education Council Award for Research Excellence 2015. The device fabrication was conducted in the microfabrication core facility of NYUAD. We would also like to thank Professor Rastislav Levicky, Department of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, Brooklyn, New York, US, for his discussion on MO-miRNA hybridization. Publisher Copyright: © 2018 Author(s).

PY - 2018/1/1

Y1 - 2018/1/1

N2 - An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.

AB - An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.

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Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip

Abstract

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