Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip

Lucia S. Cheung, Xi Wei, Diogo Martins, Yong Ak Song

Research output: Contribution to journalArticlepeer-review

Abstract

An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.

Original languageEnglish (US)
Article number014104
JournalBiomicrofluidics
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2018

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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