Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins

David Kosman; Stephen Small; John Reinitz

doi:10.1007/s004270050184

Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins

David Kosman, Stephen Small, John Reinitz

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1 2/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.

Original language	English (US)
Pages (from-to)	290-294
Number of pages	5
Journal	Development Genes and Evolution
Volume	208
Issue number	5
DOIs	https://doi.org/10.1007/s004270050184
State	Published - 1998

Keywords

Drosophila
Gene regulation
Immunological methods
Segmentation

ASJC Scopus subject areas

Genetics
Developmental Biology

Access to Document

10.1007/s004270050184

Cite this

@article{d7e38af7d3674407be7a14a435885c5f,

title = "Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins",

abstract = "We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1 2/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.",

keywords = "Drosophila, Gene regulation, Immunological methods, Segmentation",

author = "David Kosman and Stephen Small and John Reinitz",

note = "Funding Information: &p.2: wledgements This work was supported by grant RO1-RR-07801 from the NIH and grant 00014-97-1-0422 from the ONR. We thank Michael Levine for making us believe that raising serums was not exceptionally difficult. We thank Manfred Frasch and Chris Rushlow for technical advice, and we thank H. Jackle, P. MacDonald, M. Frasch, L. Pick, P. Gergen, D. Coulter, K. Cad-igan, and C. Desplan for expression constructs. We thank Leslie Pick for helpful comments on the manuscript. This is publication 259 from the Brookdale Center.",

year = "1998",

doi = "10.1007/s004270050184",

language = "English (US)",

volume = "208",

pages = "290--294",

journal = "Development Genes and Evolution",

issn = "0949-944X",

publisher = "Springer Verlag",

number = "5",

}

TY - JOUR

T1 - Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins

AU - Kosman, David

AU - Small, Stephen

AU - Reinitz, John

N1 - Funding Information: &p.2: wledgements This work was supported by grant RO1-RR-07801 from the NIH and grant 00014-97-1-0422 from the ONR. We thank Michael Levine for making us believe that raising serums was not exceptionally difficult. We thank Manfred Frasch and Chris Rushlow for technical advice, and we thank H. Jackle, P. MacDonald, M. Frasch, L. Pick, P. Gergen, D. Coulter, K. Cad-igan, and C. Desplan for expression constructs. We thank Leslie Pick for helpful comments on the manuscript. This is publication 259 from the Brookdale Center.

PY - 1998

Y1 - 1998

N2 - We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1 2/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.

AB - We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1 2/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.

KW - Drosophila

KW - Gene regulation

KW - Immunological methods

KW - Segmentation

UR - http://www.scopus.com/inward/record.url?scp=0031814029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031814029&partnerID=8YFLogxK

U2 - 10.1007/s004270050184

DO - 10.1007/s004270050184

M3 - Article

C2 - 9683745

AN - SCOPUS:0031814029

SN - 0949-944X

VL - 208

SP - 290

EP - 294

JO - Development Genes and Evolution

JF - Development Genes and Evolution

IS - 5

ER -

Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this