TY - GEN
T1 - Rapid prototyping of microfluidic probes for biomedical applications
AU - Brimmo, Ayoola T.
AU - Alnemari, Roaa
AU - Qasaimeh, Mohammad A.
N1 - Funding Information:
V. ACKNOWLEDGMENT The authors gratefully acknowledge financial support from New York University Abu Dhabi (NYUAD) and technical support from NYUAD core Technology platforms.
Publisher Copyright:
© 2018 IEEE.
PY - 2018/6/8
Y1 - 2018/6/8
N2 - The microfluidic probe (MFP) is an open space microfluidic device that combines the concepts of hydrodynamic flow confinement (HFC) and scanning probes to overcome the closed channel restrictions of conventional microfluidic devices. In biology, this allows for analysis of mammalian cells, neurons and tissue samples that are otherwise difficult to culture in conventional microfluidic devices. In this paper, we demonstrate how 3-D printing can be used to expedite the design-test cycle of the MFP and hence democratize the concept. The 3D printing procedures were adapted in fabricating the MFPs that were used for all experiments. Characterization of MFP's flow profile footprints are performed by comparisons with numerically calculated profiles. Application of the MFP is then used to selectively label adherent cells cultured in a Petri dish, within their conventional culture environment. Results show that while the 3D printed probes contain some artifacts, they function just as well as MFPs microfabricated using conventional techniques. Overall, this fabrication demonstrates a rapid, easy, and affordable fabrication technique for the MFP.
AB - The microfluidic probe (MFP) is an open space microfluidic device that combines the concepts of hydrodynamic flow confinement (HFC) and scanning probes to overcome the closed channel restrictions of conventional microfluidic devices. In biology, this allows for analysis of mammalian cells, neurons and tissue samples that are otherwise difficult to culture in conventional microfluidic devices. In this paper, we demonstrate how 3-D printing can be used to expedite the design-test cycle of the MFP and hence democratize the concept. The 3D printing procedures were adapted in fabricating the MFPs that were used for all experiments. Characterization of MFP's flow profile footprints are performed by comparisons with numerically calculated profiles. Application of the MFP is then used to selectively label adherent cells cultured in a Petri dish, within their conventional culture environment. Results show that while the 3D printed probes contain some artifacts, they function just as well as MFPs microfabricated using conventional techniques. Overall, this fabrication demonstrates a rapid, easy, and affordable fabrication technique for the MFP.
KW - 3D printing
KW - Open space microfluidic
KW - cells
KW - microfluidic probes
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U2 - 10.1109/ICASET.2018.8376894
DO - 10.1109/ICASET.2018.8376894
M3 - Conference contribution
AN - SCOPUS:85049969189
T3 - 2018 Advances in Science and Engineering Technology International Conferences, ASET 2018
SP - 1
EP - 4
BT - 2018 Advances in Science and Engineering Technology International Conferences, ASET 2018
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2018 Advances in Science and Engineering Technology International Conferences, ASET 2018
Y2 - 6 February 2018 through 5 April 2018
ER -