Rapid serotonergic fiber sprouting in response to ibotenic acid lesion in the striatum and hippocampus

Feng C. Zhou, Efrain C. Azmitia, Sharon Bledsoe

Research output: Contribution to journalArticlepeer-review


Serotonergic (5-HT) sprouting occurs after damage of neighboring 5-HT or non-5-HT fibers. The sprouting signals appear to originate from the target tissue where neural trophic factors are secreted. In order to determine the cellular source of the trophic signal we currently report that removal of local neurons with a neurotoxin, ibotenic acid (IB), triggered a vigorous sprouting of 5-HT fibers in the striatum as early as 3 days after the lesion, which lasted throughout the entire length (21 days) of our study. The 5-HT fiber density in the injection site increased 10-fold in the striatum compared to the contralateral side, and the density of 5-HT varicosities increased 4-fold of normal. The 5-HT level in the striatum correspondingly increased on the lesion side. Three clear morphological concentric zones were formed by the lesion: acellular, aneuronal and outer zones. These zones represent the characteristic reorganization of astrocytes and neurons following an injection of IB. Devoid of any cells, the small acellular zone in the center of the injection site contained few 5-HT fibers. Devoid of neurons, the aneuronal zone contained reactive astrocytes and an abnormally high density of 5-HT fibers with increased staining of S-100. Containing neurons and astrocytes, the outer zone had a normal 5-HT fiber density. The induced 5-HT sprouting in the aneuronal zone strongly suggests that local neurons could provide a trophic factor, but that astrocytes would be the most plausible candidate. This is not an isolated case, IB injections in the hippocampus also induce a similar phenomenon.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalDevelopmental Brain Research
Issue number1
StatePublished - Jan 14 1995


  • Glia
  • Neuronal plasticity
  • Neurotrophic factor
  • Reactive astrocyte
  • Regeneration
  • S-100
  • Serotonin

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology


Dive into the research topics of 'Rapid serotonergic fiber sprouting in response to ibotenic acid lesion in the striatum and hippocampus'. Together they form a unique fingerprint.

Cite this