TY - JOUR
T1 - Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein
AU - Cuomo, Christina A.
AU - Kirch, Susan A.
AU - Gyuris, Jeno
AU - Brent, Roger
AU - Oettinger, Marjorie A.
PY - 1994/6/21
Y1 - 1994/6/21
N2 - RAG1 and RAG2 are lymphoid-specific genes that together induce V(D)J recombinase activity in a variety of nonlymphoid cell types. While no other lymphoid-specific factors are required to induce recombination, other factors with more widespread expression patterns have been implicated in the reaction. However, none of these factors have been cloned, and their relationship to the RAG proteins is unclear. Using the yeast two-hybrid assay, we have identified RCH1, a gene encoding a protein of molecular weight 58,000 that interacts specifically with RAG-1. The predicted Rch1 protein sequence is 47% identical to yeast SRP1, a protein associated with the nuclear envelope. A truncated form of Rch1, which retains the ability to interact with RAG-1, reduces V(D)J recombination activity in HeLa cells.
AB - RAG1 and RAG2 are lymphoid-specific genes that together induce V(D)J recombinase activity in a variety of nonlymphoid cell types. While no other lymphoid-specific factors are required to induce recombination, other factors with more widespread expression patterns have been implicated in the reaction. However, none of these factors have been cloned, and their relationship to the RAG proteins is unclear. Using the yeast two-hybrid assay, we have identified RCH1, a gene encoding a protein of molecular weight 58,000 that interacts specifically with RAG-1. The predicted Rch1 protein sequence is 47% identical to yeast SRP1, a protein associated with the nuclear envelope. A truncated form of Rch1, which retains the ability to interact with RAG-1, reduces V(D)J recombination activity in HeLa cells.
UR - http://www.scopus.com/inward/record.url?scp=0028239241&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028239241&partnerID=8YFLogxK
M3 - Article
C2 - 8016130
AN - SCOPUS:0028239241
SN - 0027-8424
VL - 91
SP - 6156
EP - 6160
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 13
ER -