Reactivation of horseradish peroxidase in organic solvent using extraction

Jin Ryoun Kim, Yoo Seong Choi, Young Je Yoo

Research output: Contribution to journalArticlepeer-review

Abstract

A novel extraction method was developed aiming at increasing the stability of enzymes in organic solvent media. Horseradish peroxidase (HRP), inactivated in a tetrahydrofuran (THF)/water (1:1, v/v), regained and maintained its activity when HRP was extracted by adding a THF/benzene mixture to the original solution. However, the HRP activity was drastically lowered in the enzyme-free blank solution that had been formed by employing the same extraction procedure. As a result, the reactivation after the extraction is believed to depend on enzyme history, and might be arisen from an irreversible structural change of the enzyme.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number6
DOIs
StatePublished - 2000

Keywords

  • Enzyme
  • Extraction
  • Horseradish peroxidase
  • Organic solvent
  • Reactivation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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