TY - JOUR
T1 - Real-time assessment of guided bone regeneration in critical size mandibular bone defects in rats using collagen membranes with adjunct fibroblast growth factor-2
AU - Furuhata, Mitsuaki
AU - Takayama, Tadahiro
AU - Yamamoto, Takanobu
AU - Ozawa, Yasumasa
AU - Senoo, Motoki
AU - Ozaki, Manami
AU - Yamano, Seiichi
AU - Sato, Shuichi
N1 - Funding Information:
We thank the Department of Oral Health Sciences, Nihon University School of Dentistry, for technical advice and assistance, and the Department of Pathology, Nihon University School of Dentistry, for histological imaging. We also thank Prof. Yoshinori Arai who assisted and commented on the micro-CT analysis. We thank the Ito Bone Histomorphometry Institute for analyzing histological sections of newly formed bone. This research was supported in part by a Grant-in-Aid for Scientific Research (C) (No. JP19K10134 to T. Takayama) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan, Grant from Dental Research Center, Nihon University School of Dentistry (to T. Takayama), and the Sato Fund, Nihon University School of Dentistry (to T. Takayama).
Funding Information:
This research was supported in part by a Grant-in-Aid for Scientific Research (C) (No. JP19K10134 to T. Takayama) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan, Grant from Dental Research Center, Nihon University School of Dentistry (to T. Takayama), and the Sato Fund , Nihon University School of Dentistry (to T. Takayama).
Publisher Copyright:
© 2021 Association for Dental Sciences of the Republic of China
PY - 2021/10
Y1 - 2021/10
N2 - Background/purpose: Fibroblast growth factor-2 (FGF-2) regulates bone formation. The concept of guided bone regeneration using a resorbable collagen membrane (RCM) is generally accepted in implant dentistry. This study aimed to investigate the bone healing pattern in rat mandibular bone defects in real-time with and without RCM containing FGF-2 (RCM/FGF-2). Materials and methods: Critical-size circular bone defects (4.0 mm diameter) were created on both sides of the rat mandibular bone. The defects were randomly divided into the following groups: control, RCM alone, RCM containing low (0.5 μg) or high (2.0 μg) concentration of FGF-2. We performed real-time in vivo micro-computerized tomography scans at the baseline and at 2, 4, and 6 weeks, and measured the volume of newly formed bone (NFB), bone mineral density (BMD) of NFB, and the closure percentage of the NFB area. At 6 weeks, the mandibular specimens were assessed histologically and histomorphometrically to evaluate the area of new bone regeneration. Results: Real-time assessment revealed a significant increase in the volume, BMD, and closure percentage of the NFB area in the RCM/FGF-2-treated groups than that in the control and RCM groups. In the H-FGF-2 group, the volume and BMD of NFB exhibited a significant increase at 6 weeks than that at the baseline. Histological evaluation revealed the presence of osteoblasts, osteocytes, and blood vessels within the NFB. Conclusion: The real-time in vivo experiment demonstrated that RCM/FGF-2 effectively promoted bone regeneration within the critical-size mandibular defects in rats and verified new bone formation starting in the early postoperative phase.
AB - Background/purpose: Fibroblast growth factor-2 (FGF-2) regulates bone formation. The concept of guided bone regeneration using a resorbable collagen membrane (RCM) is generally accepted in implant dentistry. This study aimed to investigate the bone healing pattern in rat mandibular bone defects in real-time with and without RCM containing FGF-2 (RCM/FGF-2). Materials and methods: Critical-size circular bone defects (4.0 mm diameter) were created on both sides of the rat mandibular bone. The defects were randomly divided into the following groups: control, RCM alone, RCM containing low (0.5 μg) or high (2.0 μg) concentration of FGF-2. We performed real-time in vivo micro-computerized tomography scans at the baseline and at 2, 4, and 6 weeks, and measured the volume of newly formed bone (NFB), bone mineral density (BMD) of NFB, and the closure percentage of the NFB area. At 6 weeks, the mandibular specimens were assessed histologically and histomorphometrically to evaluate the area of new bone regeneration. Results: Real-time assessment revealed a significant increase in the volume, BMD, and closure percentage of the NFB area in the RCM/FGF-2-treated groups than that in the control and RCM groups. In the H-FGF-2 group, the volume and BMD of NFB exhibited a significant increase at 6 weeks than that at the baseline. Histological evaluation revealed the presence of osteoblasts, osteocytes, and blood vessels within the NFB. Conclusion: The real-time in vivo experiment demonstrated that RCM/FGF-2 effectively promoted bone regeneration within the critical-size mandibular defects in rats and verified new bone formation starting in the early postoperative phase.
KW - Bone regeneration
KW - Collagen membrane
KW - Fibroblast growth factor-2
KW - Growth factors
KW - Rat mandibular bone defects
KW - Real-time in vivo micro-computerized tomography
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U2 - 10.1016/j.jds.2021.03.008
DO - 10.1016/j.jds.2021.03.008
M3 - Article
AN - SCOPUS:85103708024
SN - 1991-7902
VL - 16
SP - 1170
EP - 1181
JO - Journal of Dental Sciences
JF - Journal of Dental Sciences
IS - 4
ER -