TY - JOUR
T1 - Receptor-stimulated actin polymerization requires cytoplasmic acidification in human PMNs
AU - Tonetti, Maurizio
AU - Budnick, Michael
AU - Niederman, Richard
N1 - Funding Information:
The authors wish to thank Drs. J.M. Goodson, F.E. Dewhirst and P. Stashenko for critical discussion of the manuscript. We also acknowledge the support of the National institute of Dental Research grants, DE-08415, DE-04881 and the Italian Society of Periodontics.
PY - 1990/9/1
Y1 - 1990/9/1
N2 - Cytoplasmic alkalinization has received considerable attention as a regulatory event In cell growth, transformation and signal transduction (Busa, W.B. (1986) Annu. Rev. Physiol. 48, 389-402 and Moolenar, W.H. (1986) Annu. Rev. Physiol. 48, 363-376). In contrast the current paper presents evidence for the role of an early cytoplasmic acidification, during signal transduction in the polymorphonuclear leukocyte (PMN). Following PMN stimulation with a chemotactic peptide, there is a significant decrease in cytoplasmic pH concomitant with a dramatic increase in cytoskeletal actin. The data indicate that this drop in pHi is necessary, but not sufficient, for signal transduction leading to cytoskeletal reorganization in these cells.
AB - Cytoplasmic alkalinization has received considerable attention as a regulatory event In cell growth, transformation and signal transduction (Busa, W.B. (1986) Annu. Rev. Physiol. 48, 389-402 and Moolenar, W.H. (1986) Annu. Rev. Physiol. 48, 363-376). In contrast the current paper presents evidence for the role of an early cytoplasmic acidification, during signal transduction in the polymorphonuclear leukocyte (PMN). Following PMN stimulation with a chemotactic peptide, there is a significant decrease in cytoplasmic pH concomitant with a dramatic increase in cytoskeletal actin. The data indicate that this drop in pHi is necessary, but not sufficient, for signal transduction leading to cytoskeletal reorganization in these cells.
KW - Cell movement
KW - Cytoskeleton
KW - Granulocyte
KW - Hydrogen-ion concentration
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U2 - 10.1016/0167-4889(90)90236-7
DO - 10.1016/0167-4889(90)90236-7
M3 - Article
C2 - 2400780
AN - SCOPUS:0025041540
SN - 0167-4889
VL - 1054
SP - 154
EP - 158
JO - BBA - Molecular Cell Research
JF - BBA - Molecular Cell Research
IS - 2
ER -