TY - JOUR
T1 - Recognition of Damaged DNA for Nucleotide Excision Repair
T2 - A Correlated Motion Mechanism with a Mismatched cis-syn Thymine Dimer Lesion
AU - Mu, Hong
AU - Geacintov, Nicholas E.
AU - Zhang, Yingkai
AU - Broyde, Suse
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Mammalian global genomic nucleotide excision repair requires lesion recognition by XPC, whose detailed binding mechanism remains to be elucidated. Here we have delineated the dynamic molecular pathway and energetics of lesion-specific and productive binding by the Rad4/yeast XPC lesion recognition factor, as it forms the open complex [Min, J. H., and Pavletich, N. P. (2007) Nature 449, 570-575; Chen, X., et al. (2015) Nat. Commun. 6, 5849] that is required for excision. We investigated extensively a cis-syn cyclobutane pyrimidine dimer in mismatched duplex DNA, using high-level computational approaches. Our results delineate a preferred correlated motion mechanism, which provides for the first time an atomistic description of the sequence of events as Rad4 productively binds to the damaged DNA. (Figure Presented).
AB - Mammalian global genomic nucleotide excision repair requires lesion recognition by XPC, whose detailed binding mechanism remains to be elucidated. Here we have delineated the dynamic molecular pathway and energetics of lesion-specific and productive binding by the Rad4/yeast XPC lesion recognition factor, as it forms the open complex [Min, J. H., and Pavletich, N. P. (2007) Nature 449, 570-575; Chen, X., et al. (2015) Nat. Commun. 6, 5849] that is required for excision. We investigated extensively a cis-syn cyclobutane pyrimidine dimer in mismatched duplex DNA, using high-level computational approaches. Our results delineate a preferred correlated motion mechanism, which provides for the first time an atomistic description of the sequence of events as Rad4 productively binds to the damaged DNA. (Figure Presented).
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U2 - 10.1021/acs.biochem.5b00840
DO - 10.1021/acs.biochem.5b00840
M3 - Article
C2 - 26270861
AN - SCOPUS:84940765357
SN - 0006-2960
VL - 54
SP - 5263
EP - 5267
JO - Biochemistry
JF - Biochemistry
IS - 34
ER -