Regulation of the BBB during viral encephalitis: Roles of IL-12 and NOS

Takashi Komatsu, Derek D C Ireland, Nancy Chung, Anthony Doré, Monroe Yoder, Carol Shoshkes Reiss

Research output: Contribution to journalArticlepeer-review


Intranasal infection of mice by Vesicular Stomatitis Virus (VSV) often leads to breakdown of the blood-brain barrier (BBB). The role of Interleukin 12 (IL-12) and nitric oxide synthase (NOS) was examined here. Wild-type (WT), NOS-1 knockout (KO), and NOS-3 KO mice were infected with VSV and treated with either IL-12 or medium. IL-12 treatment of uninfected hosts did not result in pathology. In contrast with WT and NOS-1 KO mice, where extensive gross and ultrastructural correlation of BBB breakdown were evident following infection, in NOS-3 KO mice, integrity of the BBB was observed. Thus NOS-3 activity in astrocytes, endothelial cells, or ependymal cells may play an essential role in regulating the BBB.

Original languageEnglish (US)
Pages (from-to)327-339
Number of pages13
JournalNitric Oxide - Biology and Chemistry
Issue number4
StatePublished - Aug 1999


  • Acquired immunity
  • Blood-brain barrier
  • Encephalitis
  • Innate immunity
  • Interleukin- 12
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research


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