TY - JOUR
T1 - Relevance of Sex and Subtype in Patients With IBS
T2 - An Exploratory Study of Gene Expression
AU - Weaver, Kristen R
AU - Melkus, Gail D'Eramo
AU - Fletcher, Jason
AU - Henderson, Wendy A
N1 - Funding Information:
The authors would like to acknowledge funding and support from the U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Nursing Research (G.D.M.: NR018075), Division of Intramural Research (W.A.H.:1ZIANR000018, 10; KRW: Intramural Research Training Award, Graduate Partnership Program). K.R.W. also received support from the National Institute of Neurological Diseases and Stroke while at Johns Hopkins University (grant no. T32NS070201) and would like to thank Conover Talbot, Jr., from the JHMI Transcriptomics and Deep Sequencing Core Facility for expression and pathway analytical assistance. Further thanks are extended to Drs. Wang, Kim, and Tamez for their input and support as part of the Intramural Program of NINR. Author Contributions Kristen R. Weaver contributed to conception and design, acquisition, analysis, and interpretation; drafted the manuscript; critically revised the manuscript; gave final approval; and agrees to be accountable for all aspects of work ensuring integrity and accuracy. Gail D’Eramo Melkus contributed to conception and design, analysis, and interpretation; critically revised the manuscript; gave final approval; and agrees to be accountable for all aspects of work ensuring integrity and accuracy. Jason Fletcher contributed to conception and design, analysis, and interpretation; critically revised the manuscript; gave final approval; and agrees to be accountable for all aspects of work ensuring integrity and accuracy. Wendy A. Henderson contributed to conception and design, acquisition, analysis, and interpretation; critically revised the manuscript; gave final approval; and agrees to be accountable for all aspects of work ensuring integrity and accuracy. Declaration of Conflicting Interests The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Funding The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Nursing Research (G.D.M.: NR018075), Division of Intramural Research (W.A.H.:1ZIANR000018, 10; K.R.W.: Intramural Research Training Award, Graduate Partnership Program). K.R.W. also received support from the National Institute of Neurological Diseases and Stroke while at Johns Hopkins University (grant no. T32NS070201). ORCID iDs Kristen R. Weaver https://orcid.org/0000-0002-8757-8758 Wendy A. Henderson https://orcid.org/0000-0003-3924-7118
Funding Information:
The authors would like to acknowledge funding and support from the U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Nursing Research (G.D.M.: NR018075), Division of Intramural Research (W.A.H.:1ZIANR000018, 10; KRW: Intramural Research Training Award, Graduate Partnership Program). K.R.W. also received support from the National Institute of Neurological Diseases and Stroke while at Johns Hopkins University (grant no. T32NS070201) and would like to thank Conover Talbot, Jr., from the JHMI Transcriptomics and Deep Sequencing Core Facility for expression and pathway analytical assistance. Further thanks are extended to Drs. Wang, Kim, and Tamez for their input and support as part of the Intramural Program of NINR. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Nursing Research (G.D.M.: NR018075), Division of Intramural Research (W.A.H.:1ZIANR000018, 10; K.R.W.: Intramural Research Training Award, Graduate Partnership Program). K.R.W. also received support from the National Institute of Neurological Diseases and Stroke while at Johns Hopkins University (grant no. T32NS070201).
Publisher Copyright:
© The Author(s) 2019.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - BACKGROUND: Psychological state, stress level, and gastrointestinal function are intricately related and relevant to symptom exacerbation in patients with irritable bowel syndrome (IBS), but genetic contributors to this brain-gut connection are not fully understood. The purpose of this exploratory study was to compare gene expression in participants with IBS to that of healthy controls (HC) and to examine patterns of expression in participants with IBS by sex and IBS subtype.METHOD: Participants were recruited to an ongoing protocol at the National Institutes of Health. Differences in demographic and clinical characteristics were assessed using descriptive statistics and Mann-Whitney U tests. Expression levels of 84 genes were evaluated in peripheral whole blood using Custom RT2 Profiler polymerase chain reaction (PCR) Arrays, and data analysis was performed through GeneGlobe Data Analysis Center.RESULTS: Participants with IBS (n = 27) reported greater levels of perceived stress (p = .037) and differed in expression values of ±2 for the genes ADIPOR1, ADIPOR2, CNR2, COMT, OXTR, and PPARA compared to HC (n = 43). Further analyses by sex and IBS subtype revealed differential patterns of gene expression related to the endocannabinoid system, cytokines, stress, and sex steroid hormones.CONCLUSIONS: Diverse yet interconnected processes such as metabolism, inflammation, immunity, social behavior, and pain are associated with differences in gene expression between participants with IBS and HC. These findings lend support for genomic associations with the brain-gut connection in patients with IBS and highlight the relevance of sex and IBS subtype in performing such analyses.
AB - BACKGROUND: Psychological state, stress level, and gastrointestinal function are intricately related and relevant to symptom exacerbation in patients with irritable bowel syndrome (IBS), but genetic contributors to this brain-gut connection are not fully understood. The purpose of this exploratory study was to compare gene expression in participants with IBS to that of healthy controls (HC) and to examine patterns of expression in participants with IBS by sex and IBS subtype.METHOD: Participants were recruited to an ongoing protocol at the National Institutes of Health. Differences in demographic and clinical characteristics were assessed using descriptive statistics and Mann-Whitney U tests. Expression levels of 84 genes were evaluated in peripheral whole blood using Custom RT2 Profiler polymerase chain reaction (PCR) Arrays, and data analysis was performed through GeneGlobe Data Analysis Center.RESULTS: Participants with IBS (n = 27) reported greater levels of perceived stress (p = .037) and differed in expression values of ±2 for the genes ADIPOR1, ADIPOR2, CNR2, COMT, OXTR, and PPARA compared to HC (n = 43). Further analyses by sex and IBS subtype revealed differential patterns of gene expression related to the endocannabinoid system, cytokines, stress, and sex steroid hormones.CONCLUSIONS: Diverse yet interconnected processes such as metabolism, inflammation, immunity, social behavior, and pain are associated with differences in gene expression between participants with IBS and HC. These findings lend support for genomic associations with the brain-gut connection in patients with IBS and highlight the relevance of sex and IBS subtype in performing such analyses.
KW - IBS subtype
KW - gene expression
KW - irritable bowel syndrome
KW - sex differences
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U2 - 10.1177/1099800419889189
DO - 10.1177/1099800419889189
M3 - Article
C2 - 31833409
SN - 1099-8004
VL - 22
SP - 13
EP - 23
JO - Biological Research for Nursing
JF - Biological Research for Nursing
IS - 1
ER -