Remineralization effect of CPP-ACP and fluoride for white spot lesions in vitro

Gustavo M.S. Oliveira, André V. Ritter, Harald O. Heymann, Edward Swift, Terry Donovan, Guy Brock, Tim Wright

Research output: Contribution to journalArticlepeer-review


Objectives This in vitro study compared the remineralization effect on white spot lesions of casein phosphopeptide-amorphous calcium phosphate crème, or CPP-ACP (MI Paste™), 1.1% NaF dentifrice containing 5000 ppm of fluoride (ControlRX™), or CPP-ACP crème with 900 ppm of fluoride (MI Paste Plus™) with that of a control.

Methods Artificial white spot lesions were created on smooth enamel surfaces of sound molars using a previously reported demineralization model. Specimens were randomly assigned to four treatments (n = 35) with a pH-cycling model over 30 days: Control (no treatment); MI Paste (10% CPP-ACP crème); F5000 (1.1% NaF dentifrice); or MI Paste Plus (10% CPP-ACP plus 900 ppm fluoride crème). Products were applied following manufacturers' directions. Changes in mean lesion depth expressed by percent fluorescence loss (ΔF%), and lesion area (mm2) from baseline to after treatment were measured with light-induced fluorescence (QLF). Mean values of each parameter were compared between groups (p < 0.05).

Results The remineralization pattern for the F5000 group was unique with marked initial remineralization during the first 10 days and little subsequent change. Based on mean lesion area, the F5000 demonstrated greater remineralization than Control, MI Paste and MI Paste Plus groups. Based on mean fluorescence loss, the F5000 group showed improved remineralization relative to MI Paste Plus, but did not differ statistically from the Control at the end of 30 days.

Conclusions The 1.1% NaF dentifrice demonstrated overall greater remineralization ability than 10% CPP-ACP crème. However, the 1.1% NaF dentifrice was only as effective as the Control to reduce fluorescence loss.

Clinical significance This study showed that a 1.1% NaF dentifrice (5000 ppm) demonstrated greater remineralization ability than the CPP-ACP topical tooth crème and that the addition of fluoride to its formulation seems to enhance remineralization. Saliva also has the ability to exert an important remineralization effect over time.

Original languageEnglish (US)
Pages (from-to)1592-1602
Number of pages11
JournalJournal of Dentistry
Issue number12
StatePublished - Dec 1 2014


  • Fluoride
  • QLF
  • Remineralization
  • White spot lesions

ASJC Scopus subject areas

  • General Dentistry


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