TY - JOUR
T1 - Removal of FKBP12 Enhances mTOR-Raptor Interactions, LTP, Memory, and Perseverative/Repetitive Behavior
AU - Hoeffer, Charles A.
AU - Tang, Wei
AU - Wong, Helen
AU - Santillan, Arturo
AU - Patterson, Richard J.
AU - Martinez, Luis A.
AU - Tejada-Simon, Maria V.
AU - Paylor, Richard
AU - Hamilton, Susan L.
AU - Klann, Eric
N1 - Funding Information:
We thank Drs. Jonathan Levenson, Faridis Serrano, Lingfei Hou, Caterina Hernandez, Corrine Spencer, Paula Aracena, and J. Lebowski for providing reagents and expert advice. We also thank the Baylor College of Medicine MRDDRC Neurobehavioral Core (HD24064) for expert technical assistance. This work was supported by NIH grants NS048037 and NS034007 (E.K.), T32 grant HL07676 (C.A.H.), and AR41802 (S.L.H.).
PY - 2008/12/10
Y1 - 2008/12/10
N2 - FK506-binding protein 12 (FKBP12) binds the immunosuppressant drugs FK506 and rapamycin and regulates several signaling pathways, including mammalian target of rapamycin (mTOR) signaling. We determined whether the brain-specific disruption of the FKBP12 gene in mice altered mTOR signaling, synaptic plasticity, and memory. Biochemically, the FKBP12-deficient mice displayed increases in basal mTOR phosphorylation, mTOR-Raptor interactions, and p70 S6 kinase (S6K) phosphorylation. Electrophysiological experiments revealed that FKBP12 deficiency was associated with an enhancement in long-lasting hippocampal long-term potentiation (LTP). The LTP enhancement was resistant to rapamycin, but not anisomycin, suggesting that altered translation control is involved in the enhanced synaptic plasticity. Behaviorally, FKBP12 conditional knockout (cKO) mice displayed enhanced contextual fear memory and autistic/obsessive-compulsive-like perseveration in several assays including the water maze, Y-maze reversal task, and the novel object recognition test. Our results indicate that FKBP12 plays a critical role in the regulation of mTOR-Raptor interactions, LTP, memory, and perseverative behaviors.
AB - FK506-binding protein 12 (FKBP12) binds the immunosuppressant drugs FK506 and rapamycin and regulates several signaling pathways, including mammalian target of rapamycin (mTOR) signaling. We determined whether the brain-specific disruption of the FKBP12 gene in mice altered mTOR signaling, synaptic plasticity, and memory. Biochemically, the FKBP12-deficient mice displayed increases in basal mTOR phosphorylation, mTOR-Raptor interactions, and p70 S6 kinase (S6K) phosphorylation. Electrophysiological experiments revealed that FKBP12 deficiency was associated with an enhancement in long-lasting hippocampal long-term potentiation (LTP). The LTP enhancement was resistant to rapamycin, but not anisomycin, suggesting that altered translation control is involved in the enhanced synaptic plasticity. Behaviorally, FKBP12 conditional knockout (cKO) mice displayed enhanced contextual fear memory and autistic/obsessive-compulsive-like perseveration in several assays including the water maze, Y-maze reversal task, and the novel object recognition test. Our results indicate that FKBP12 plays a critical role in the regulation of mTOR-Raptor interactions, LTP, memory, and perseverative behaviors.
KW - HUMDISEASE
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U2 - 10.1016/j.neuron.2008.09.037
DO - 10.1016/j.neuron.2008.09.037
M3 - Article
C2 - 19081378
AN - SCOPUS:57049114322
SN - 0896-6273
VL - 60
SP - 832
EP - 845
JO - Neuron
JF - Neuron
IS - 5
ER -