Removal of FKBP12 Enhances mTOR-Raptor Interactions, LTP, Memory, and Perseverative/Repetitive Behavior

Charles A. Hoeffer, Wei Tang, Helen Wong, Arturo Santillan, Richard J. Patterson, Luis A. Martinez, Maria V. Tejada-Simon, Richard Paylor, Susan L. Hamilton, Eric Klann

Research output: Contribution to journalArticle

Abstract

FK506-binding protein 12 (FKBP12) binds the immunosuppressant drugs FK506 and rapamycin and regulates several signaling pathways, including mammalian target of rapamycin (mTOR) signaling. We determined whether the brain-specific disruption of the FKBP12 gene in mice altered mTOR signaling, synaptic plasticity, and memory. Biochemically, the FKBP12-deficient mice displayed increases in basal mTOR phosphorylation, mTOR-Raptor interactions, and p70 S6 kinase (S6K) phosphorylation. Electrophysiological experiments revealed that FKBP12 deficiency was associated with an enhancement in long-lasting hippocampal long-term potentiation (LTP). The LTP enhancement was resistant to rapamycin, but not anisomycin, suggesting that altered translation control is involved in the enhanced synaptic plasticity. Behaviorally, FKBP12 conditional knockout (cKO) mice displayed enhanced contextual fear memory and autistic/obsessive-compulsive-like perseveration in several assays including the water maze, Y-maze reversal task, and the novel object recognition test. Our results indicate that FKBP12 plays a critical role in the regulation of mTOR-Raptor interactions, LTP, memory, and perseverative behaviors.

Original languageEnglish (US)
Pages (from-to)832-845
Number of pages14
JournalNeuron
Volume60
Issue number5
DOIs
StatePublished - Dec 10 2008

Keywords

  • HUMDISEASE

ASJC Scopus subject areas

  • Neuroscience(all)

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    Hoeffer, C. A., Tang, W., Wong, H., Santillan, A., Patterson, R. J., Martinez, L. A., Tejada-Simon, M. V., Paylor, R., Hamilton, S. L., & Klann, E. (2008). Removal of FKBP12 Enhances mTOR-Raptor Interactions, LTP, Memory, and Perseverative/Repetitive Behavior. Neuron, 60(5), 832-845. https://doi.org/10.1016/j.neuron.2008.09.037