TY - JOUR
T1 - Resolution of branched DNA substrates by T7 endonuclease I and its inhibition
AU - Lu, Min
AU - Guo, Qiu
AU - Studier, F. William
AU - Kallenbach, Neville R.
PY - 1991/2/5
Y1 - 1991/2/5
N2 - Endonuclease I is a multipurpose enzyme implicated in the breakdown of host DNA, packaging of phage DNA, and recombination during the lytic cycle of bacteriophage T7. We investigate here some aspects of the substrate requirements for its activity in resolving branched intermediates similar to Holliday junctions (Holliday, R. (1964) Genet. Res. 5, 282-304) that arise in recombination. The enzyme is able to resolve branched substrates containing very short duplex arms: 4 base pairs suffice. It cleaves 5′ to the branch, with a distinct preference for the non-crossover strands in Holliday-like model junctions. Ligands that interact strongly with the branch site can inhibit the enzyme, with KI values in the 10-50 μM range.
AB - Endonuclease I is a multipurpose enzyme implicated in the breakdown of host DNA, packaging of phage DNA, and recombination during the lytic cycle of bacteriophage T7. We investigate here some aspects of the substrate requirements for its activity in resolving branched intermediates similar to Holliday junctions (Holliday, R. (1964) Genet. Res. 5, 282-304) that arise in recombination. The enzyme is able to resolve branched substrates containing very short duplex arms: 4 base pairs suffice. It cleaves 5′ to the branch, with a distinct preference for the non-crossover strands in Holliday-like model junctions. Ligands that interact strongly with the branch site can inhibit the enzyme, with KI values in the 10-50 μM range.
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M3 - Article
C2 - 1990002
AN - SCOPUS:0025850530
SN - 0021-9258
VL - 266
SP - 2531
EP - 2536
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 4
ER -