TY - JOUR
T1 - Response of stem cells from different origins to biphasic calcium phosphate bioceramics
AU - Lobo, Sonja E.
AU - Glickman, Robert
AU - da Silva, Wagner N.
AU - Arinzeh, Treena L.
AU - Kerkis, Irina
N1 - Funding Information:
The authors pay tribute to the late Dr Racquel LeGeros, in whose laboratory part of this study was performed; her friendship, guidance and legacy will be remembered forever. This study was supported by EINCO Biomaterial Ltda (Belo Horizonte, Minas Gerais, Brazil) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), Brazil.
Publisher Copyright:
© 2015, The Author(s).
PY - 2015/8/10
Y1 - 2015/8/10
N2 - Biphasic calcium phosphate (BCP) bioceramics have been successfully applied in a broad variety of presentation forms and with different ratios of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). BCPs have been loaded with stem cells from different origins for bone tissue engineering purposes, but evidence of stem cell behavior on different compositions (various HA/β-TCP ratios) and physical features of BCPs is limited. We compared the adhesion, proliferation, viability and osteogenic potential of human mesenchymal stem cells (MSCs) on granular BCPs with equal HA/β-TCP ratio of diverse particle sizes and on porous blocks which had different chemical compositions. In addition, the osteogenic differentiation of MSCs was compared to adipose-derived (ADSC) and dental pulp (DPSC) stem cells, as well as to pre-osteoblasts on a particulate BCP. MSCs growing on granular BCPs demonstrated increased number as compared to MSCs growing on blocks. Cells proliferated to a greater extent on small granular BCPs, while large granular BCPs and blocks promoted cell differentiation. Surprisingly, the expression of genes involved in osteogenesis was upregulated in MSCs on bioceramics in basal medium which indicates that BCPs may have osteoinductive potential. This was confirmed with the upregulation of osteochondrogenic markers, at different time points, when stem cells from various tissues were grown on the BCP. This study demonstrates that BCPs, depending on their physical features and chemical composition, modulate stem cell behavior, and that stem cells from different origins are inherently distinct in their gene expression profile and can be triggered toward osteochondrogenic fate by BCPs.
AB - Biphasic calcium phosphate (BCP) bioceramics have been successfully applied in a broad variety of presentation forms and with different ratios of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). BCPs have been loaded with stem cells from different origins for bone tissue engineering purposes, but evidence of stem cell behavior on different compositions (various HA/β-TCP ratios) and physical features of BCPs is limited. We compared the adhesion, proliferation, viability and osteogenic potential of human mesenchymal stem cells (MSCs) on granular BCPs with equal HA/β-TCP ratio of diverse particle sizes and on porous blocks which had different chemical compositions. In addition, the osteogenic differentiation of MSCs was compared to adipose-derived (ADSC) and dental pulp (DPSC) stem cells, as well as to pre-osteoblasts on a particulate BCP. MSCs growing on granular BCPs demonstrated increased number as compared to MSCs growing on blocks. Cells proliferated to a greater extent on small granular BCPs, while large granular BCPs and blocks promoted cell differentiation. Surprisingly, the expression of genes involved in osteogenesis was upregulated in MSCs on bioceramics in basal medium which indicates that BCPs may have osteoinductive potential. This was confirmed with the upregulation of osteochondrogenic markers, at different time points, when stem cells from various tissues were grown on the BCP. This study demonstrates that BCPs, depending on their physical features and chemical composition, modulate stem cell behavior, and that stem cells from different origins are inherently distinct in their gene expression profile and can be triggered toward osteochondrogenic fate by BCPs.
KW - Adipose-derived stem cells
KW - Dental pulp stem cells
KW - Hydroxyapatite
KW - Mesenchymal stem cells
KW - β-tricalcium phosphate
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U2 - 10.1007/s00441-015-2116-9
DO - 10.1007/s00441-015-2116-9
M3 - Article
C2 - 25676006
AN - SCOPUS:84938746510
SN - 0302-766X
VL - 361
SP - 477
EP - 495
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 2
ER -