Retinoid X receptor-selective ligands produce malformations in Xenopus embryos

Saverio Minucci, Jean Pierre Saint-Jeannet, Reiko Toyama, Giorgio Scita, Luigi M. Deluca, Masanori Taira, Arthur A. Levin, Keiko Ozato, Igor B. Dawid

Research output: Contribution to journalArticlepeer-review


Retinoids exert pleiotropic effects on the development of vertebrates through the action of retinoic acid receptors (RAR) and retinoid X receptors (RXR). We have investigated the effect of synthetic retinoids selective for RXR and RAR on the development of Xenopus and zebrafish embryos. In Xenopus, both ligands selective for RAR and RXR caused striking malformations along the anterior-posterior axis, whereas in zebrafish only ligands specific for RAR caused embryonic malformations. In Xenopus, RAR- and RXR-selective ligands regulated the expression of the Xlim-1, gsc, and HoxA1 genes similarly as all-trans-retinoic acid. Nevertheless, RXR-selective ligands activated only an RXR responsive reporter but not an RAR responsive reporter introduced by microinjection into the Xenopus embryo, consistent with our failure to detect conversion of an RXR-selective ligand to different derivatives in the embryo. These results suggest that Xenopus embryos possess a unique response pathway in which liganded RXR can control gene expression. Our observations further illustrate the divergence in retinoid responsiveness between different vertebrate species.

Original languageEnglish (US)
Pages (from-to)1803-1807
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number5
StatePublished - Mar 5 1996


  • Xenopus laevis
  • retinoic acid receptor
  • retinoids
  • zebrafish

ASJC Scopus subject areas

  • General


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