Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription

Qing Huang, Katarzyna J. Purzycka, Sabrina Lusvarghi, Donghui Li, Stuart F.J. Legrice, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    Ty1 retrotransposon RNA has the potential to fold into a variety of distinct structures, mutation of which affects retrotransposition frequencies. We show here that one potential functional structure is located at the 5' end of the genome and can assume a pseudoknot conformation. Chemoenzymatic probing of wild-type and mutant mini-Ty1 RNAs supports the existence of such a structure, while molecular genetic analyses show that mutations disrupting pseudoknot formation interfere with retrotransposition, indicating that it provides a critical biological function. These defects are enhanced at higher temperatures. When these mutants are combined with compensatory changes, retrotransposition is restored, consistent with pseudoknot architecture. Analyses of mutants suggest a defect in Ty1 reverse transcription. Collectively, our data allow modeling of a three-dimensional structure for this novel critical cis-acting signal of the Ty1 genome.

    Original languageEnglish (US)
    Pages (from-to)320-322
    Number of pages3
    JournalRNA
    Volume19
    Issue number3
    DOIs
    StatePublished - Mar 2013

    Keywords

    • Pseudoknot
    • RNA structure
    • Retrotransposon
    • Reverse transcription
    • SHAPE

    ASJC Scopus subject areas

    • Molecular Biology

    Fingerprint Dive into the research topics of 'Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription'. Together they form a unique fingerprint.

  • Cite this

    Huang, Q., Purzycka, K. J., Lusvarghi, S., Li, D., Legrice, S. F. J., & Boeke, J. D. (2013). Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription. RNA, 19(3), 320-322. https://doi.org/10.1261/rna.035535.112