Reverse transcriptase encoded by a human transposable element

Stephen L. Mathias, Alan F. Scott, Haig H. Kazazian, Jef D. Boeke, Abram Gabriel

    Research output: Contribution to journalArticle

    Abstract

    L1 elements are highly repeated mammalian DNA sequences whose structure suggests dispersal by retrotransposition. A consensus L1 element encodes a protein with sequence similarity to known reverse transcriptases. The second open reading frame from the human L1 element L1.2A was expressed as a fusion protein targeted to Tyl virus-like particles in Saccharomyces cerevisiae and shown to have reverse transcriptase activity. This activity was eliminated by a missense mutation in the highly conserved amino acid motif Y/F-X-D-D. Thus, L1 represents a potential source of the reverse transcriptase activity necessary for dispersion of the many classes of mammalian retroelements.

    Original languageEnglish (US)
    Pages (from-to)1808-1810
    Number of pages3
    JournalScience
    Volume254
    Issue number5039
    DOIs
    StatePublished - 1991

    ASJC Scopus subject areas

    • General

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  • Cite this

    Mathias, S. L., Scott, A. F., Kazazian, H. H., Boeke, J. D., & Gabriel, A. (1991). Reverse transcriptase encoded by a human transposable element. Science, 254(5039), 1808-1810. https://doi.org/10.1126/science.1722352