Reversed phase ion-pairing chromatography of an oligolysine mixture in different mobile phases: Effort of searching critical chromatography conditions

Wenchun Xie, Iwao Teraoka, Richard A. Gross

Research output: Contribution to journalArticlepeer-review

Abstract

Our earlier study [J. Chromatogr. A 1218 (2011) 7765] on separation of an oligolysine mixture consisting of chains with 2-8 lysine residues (number of lysine residues, dp. = 2-8) by ion-pairing reversed-phase chromatography using heptafluorobutyric acid (HFBA) as an ion pairing reagent at fixed mobile phase acetonitrile (ACN) content was extended to isocratic elution conditions with different ACN percentages. The present work explored how manipulating the mobile phase HFBA concentration ([HFBA]) and %-ACN content influences separations of the oligolysine mixture. The closed pairing model was used to analyze variation of the retention factor as a function of [HFBA]. The partition coefficient of the paired peptide decreased with increasing %-ACN. Pairing of HFBA to oligolysine was cooperative, and the effect increased when %-ACN in the mobile phase was lowered. A plot of the partition coefficient as a function of %-ACN for oligolysines varying in dp converged at one ACN content, indicating a critical condition in which components of different dp co-elute.

Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalJournal of Chromatography A
Volume1304
DOIs
StatePublished - Aug 23 2013

Keywords

  • Critical chromatography condition
  • Heptafluorobutyric acid
  • Ion-pairing chromatography
  • Mobile phase composition
  • Oligolysine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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