Reversible optical control of F1Fo-ATP synthase using photoswitchable inhibitors

Bianca Eisel, Felix W.W. Hartrampf, Thomas Meier, Dirk Trauner

Research output: Contribution to journalLetter

Abstract

F1Fo-ATP synthase is one of the best studied macromolecular machines in nature. It can be inhibited by a range of small molecules, which include the polyphenols, resveratrol and piceatannol. Here, we introduce Photoswitchable Inhibitors of ATP Synthase, termed PIAS, which were synthetically derived from these polyphenols. They can be used to reversibly control the enzymatic activity of purified yeast Yarrowia lipolyticaATP synthase by light. Our experiments indicate that the PIAS bind to the same site in the ATP synthase F1 complex as the polyphenols in their trans form, but they do not bind in their cis form. The PIAS could be useful tools for the optical precision control of ATP synthase in a variety of biochemical and biotechnological applications.

Original languageEnglish (US)
Pages (from-to)343-355
Number of pages13
JournalFEBS Letters
Volume592
Issue number3
DOIs
StatePublished - Feb 2018

Keywords

  • Yarrowia lipolytica FF-ATP synthase
  • photopharmacology

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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