TY - JOUR
T1 - RNA localization is a key determinant of neurite-enriched proteome
AU - Zappulo, Alessandra
AU - Van Den Bruck, David
AU - Ciolli Mattioli, Camilla
AU - Franke, Vedran
AU - Imami, Koshi
AU - McShane, Erik
AU - Moreno-Estelles, Mireia
AU - Calviello, Lorenzo
AU - Filipchyk, Andrei
AU - Peguero-Sanchez, Esteban
AU - Müller, Thomas
AU - Woehler, Andrew
AU - Birchmeier, Carmen
AU - Merino, Enrique
AU - Rajewsky, Nikolaus
AU - Ohler, Uwe
AU - Mazzoni, Esteban O.
AU - Selbach, Matthias
AU - Akalin, Altuna
AU - Chekulaeva, Marina
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Protein subcellular localization is fundamental to the establishment of the body axis, cell migration, synaptic plasticity, and a vast range of other biological processes. Protein localization occurs through three mechanisms: protein transport, mRNA localization, and local translation. However, the relative contribution of each process to neuronal polarity remains unknown. Using neurons differentiated from mouse embryonic stem cells, we analyze protein and RNA expression and translation rates in isolated cell bodies and neurites genome-wide. We quantify 7323 proteins and the entire transcriptome, and identify hundreds of neurite-localized proteins and locally translated mRNAs. Our results demonstrate that mRNA localization is the primary mechanism for protein localization in neurites that may account for half of the neurite-localized proteome. Moreover, we identify multiple neurite-targeted non-coding RNAs and RNA-binding proteins with potential regulatory roles. These results provide further insight into the mechanisms underlying the establishment of neuronal polarity.
AB - Protein subcellular localization is fundamental to the establishment of the body axis, cell migration, synaptic plasticity, and a vast range of other biological processes. Protein localization occurs through three mechanisms: protein transport, mRNA localization, and local translation. However, the relative contribution of each process to neuronal polarity remains unknown. Using neurons differentiated from mouse embryonic stem cells, we analyze protein and RNA expression and translation rates in isolated cell bodies and neurites genome-wide. We quantify 7323 proteins and the entire transcriptome, and identify hundreds of neurite-localized proteins and locally translated mRNAs. Our results demonstrate that mRNA localization is the primary mechanism for protein localization in neurites that may account for half of the neurite-localized proteome. Moreover, we identify multiple neurite-targeted non-coding RNAs and RNA-binding proteins with potential regulatory roles. These results provide further insight into the mechanisms underlying the establishment of neuronal polarity.
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U2 - 10.1038/s41467-017-00690-6
DO - 10.1038/s41467-017-00690-6
M3 - Article
C2 - 28928394
AN - SCOPUS:85029833281
SN - 2041-1723
VL - 8
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 583
ER -