RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

Mikel Zaratiegui; Stephane E. Castel; Danielle V. Irvine; Anna Kloc; Jie Ren; Fei Li; Elisa De Castro; Laura Marín; An Yun Chang; Derek Goto; W. Zacheus Cande; Francisco Antequera; Benoit Arcangioli; Robert A. Martienssen

doi:10.1038/nature10501

RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

Mikel Zaratiegui, Stephane E. Castel, Danielle V. Irvine, Anna Kloc, Jie Ren, Fei Li, Elisa De Castro, Laura Marín, An Yun Chang, Derek Goto, W. Zacheus Cande, Francisco Antequera, Benoit Arcangioli, Robert A. Martienssen

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.

Original language	English (US)
Pages (from-to)	135-138
Number of pages	4
Journal	Nature
Volume	479
Issue number	7371
DOIs	https://doi.org/10.1038/nature10501
State	Published - Nov 3 2011

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@article{9195775d344e4c8a8c1eabac8b24cdd3,

title = "RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II",

abstract = "Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.",

author = "Mikel Zaratiegui and Castel, {Stephane E.} and Irvine, {Danielle V.} and Anna Kloc and Jie Ren and Fei Li and {De Castro}, Elisa and Laura Mar{\'i}n and Chang, {An Yun} and Derek Goto and Cande, {W. Zacheus} and Francisco Antequera and Benoit Arcangioli and Martienssen, {Robert A.}",

note = "Funding Information: Acknowledgements We thank D. Roh and T. Volpe for isolating cDNA clones. D.V.I. was supported by a NHMRC CJ Martin Postdoctoral Research Fellowship. M.Z. was supported by a fellowship from the Spanish Ministry of Science. This work was supported by grants BFU2008-01919 and Consolider-Ingenio CSD2007-00015 from the Spanish Ministry of Science and Innovation to F.A., and NIH R01 GM076396 to W.Z.C. and R.A.M.",

year = "2011",

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doi = "10.1038/nature10501",

language = "English (US)",

volume = "479",

pages = "135--138",

journal = "Nature",

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TY - JOUR

T1 - RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

AU - Zaratiegui, Mikel

AU - Castel, Stephane E.

AU - Irvine, Danielle V.

AU - Kloc, Anna

AU - Ren, Jie

AU - Li, Fei

AU - De Castro, Elisa

AU - Marín, Laura

AU - Chang, An Yun

AU - Goto, Derek

AU - Cande, W. Zacheus

AU - Antequera, Francisco

AU - Arcangioli, Benoit

AU - Martienssen, Robert A.

N1 - Funding Information: Acknowledgements We thank D. Roh and T. Volpe for isolating cDNA clones. D.V.I. was supported by a NHMRC CJ Martin Postdoctoral Research Fellowship. M.Z. was supported by a fellowship from the Spanish Ministry of Science. This work was supported by grants BFU2008-01919 and Consolider-Ingenio CSD2007-00015 from the Spanish Ministry of Science and Innovation to F.A., and NIH R01 GM076396 to W.Z.C. and R.A.M.

PY - 2011/11/3

Y1 - 2011/11/3

N2 - Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.

AB - Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.

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JF - Nature

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ER -

RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

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