Runx2, a target gene for activating transcription factor-3 in human breast cancer cells

M. Gokulnath, N. C. Partridge, N. Selvamurugan

Research output: Contribution to journalArticlepeer-review

Abstract

Activating transcription factor (ATF-3) is a stress response gene and is induced by transforming growth factor beta 1 (TGF-β1) in breast cancer cells. In this study, we dissected the functional role of ATF-3 gene in vitro by knocking down its expression stably in human bone metastatic breast cancer cells (MDA-MB231). Knockdown of ATF-3 expression in these cells decreased cell number, altered cell cycle phase transition, and decreased mRNA expression of cell cycle genes. Knockdown of ATF-3 expression in MDA-MB231 cells also decreased cell migration, and the expression levels of invasive and metastatic genes such as MMP-13 and Runx2 were found to be decreased in these cells. Most importantly, ATF-3 was associated with Runx2 promoter in MDA-MB231 cells and knockdown of ATF-3 expression decreased its association with Runx2 promoter. Hence, our results suggested that ATF-3 plays a role in proliferation and invasion of bone metastatic breast cancer cells in vitro and we identified for the first time that Runx2 is a target gene of ATF-3 in MDA-MB231 cell line.

Original languageEnglish (US)
Pages (from-to)1923-1931
Number of pages9
JournalTumor Biology
Volume36
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • ATF-3
  • Bone metastasis
  • MMP-13
  • Runx2
  • TGF-β1

ASJC Scopus subject areas

  • Cancer Research

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