SAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathway

Okunola Jeyifous, Clarissa L. Waites, Christian G. Specht, Sho Fujisawa, Manja Schubert, Eric I. Lin, John Marshall, Chiye Aoki, Tharani De Silva, Johanna M. Montgomery, Craig C. Garner, William N. Green

Research output: Contribution to journalArticlepeer-review


Synaptic plasticity is dependent on the differential sorting, delivery and retention of neurotransmitter receptors, but the mechanisms underlying these processes are poorly understood. We found that differential sorting of glutamate receptor subtypes began in the endoplasmic reticulum of rat hippocampal neurons. As AMPA receptors (AMPARs) were trafficked to the plasma membrane via the conventional somatic Golgi network, NMDA receptors (NMDARs) were diverted from the somatic endoplasmic reticulum into a specialized endoplasmic reticulum subcompartment that bypasses somatic Golgi, merging instead with dendritic Golgi outposts. This endoplasmic reticulum subcompartment was composed of highly mobile vesicles containing the NMDAR subunits NR1 and NR2B, the microtubule-dependent motor protein KIF17, and the postsynaptic adaptor proteins CASK and SAP97. Our data demonstrate that the retention and trafficking of NMDARs in this endoplasmic reticulum subcompartment requires both CASK and SAP97. These findings indicate that NMDARs are sorted away from AMPARs via a non-conventional secretory pathway that utilizes dendritic Golgi outposts.

Original languageEnglish (US)
Pages (from-to)1011-1019
Number of pages9
JournalNature Neuroscience
Issue number8
StatePublished - Aug 2009

ASJC Scopus subject areas

  • General Neuroscience


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