SDH1, the gene encoding the succinate dehydrogenase flavoprotein subunit from Saccharomyces cerevisiae

Karen B. Chapman, Sharon D. Solomon, Jef D. Boeke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We describe the isolation, sequence and construction of a disruption of the yeast SDH1 gene, encoding the flavoprotein subunit of succinate dehydrogenase. This is the first eukaryotic flavoprotein subunit-encoding gene to be fully sequenced. The deduced amino acid (aa) sequence is 50% identical to the Escherichia coli enzyme sequence. The yeast gene encodes an N-terminal extension of 45 aa relative to the E. coli sequence which may act as a mitochondrial targeting signal. Disruption of the gene results in the inability to respire, assayed as the inability to utilize the nonfermentable carbon source, glycerol. This is the expected phenotype for disruption of an essential component of the yeast citric acid cycle.

    Original languageEnglish (US)
    Pages (from-to)131-136
    Number of pages6
    JournalGene
    Volume118
    Issue number1
    DOIs
    StatePublished - Sep 1 1992

    Keywords

    • Nucleotide sequence
    • citric acid cycle
    • electron transport
    • mitochondrial proteins
    • petite mutants

    ASJC Scopus subject areas

    • Genetics

    Fingerprint Dive into the research topics of 'SDH1, the gene encoding the succinate dehydrogenase flavoprotein subunit from Saccharomyces cerevisiae'. Together they form a unique fingerprint.

    Cite this