Selective effect of inhibitors on inner mitochondrial membrane channels

Yuri N. Antonenko; Kathleen W. Kinnally; Sean Perini; Henry Tedeschi

doi:10.1016/0014-5793(91)80731-H

Selective effect of inhibitors on inner mitochondrial membrane channels

Yuri N. Antonenko, Kathleen W. Kinnally, Sean Perini, Henry Tedeschi

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20% (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.

Original language	English (US)
Pages (from-to)	89-93
Number of pages	5
Journal	FEBS Letters
Volume	285
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(91)80731-H
State	Published - Jul 8 1991

Keywords

Inner mitochondrial membrane
Mitochondrial channel
Patch-clamp
Pharmacology

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80731-H

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title = "Selective effect of inhibitors on inner mitochondrial membrane channels",

abstract = "The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20% (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.",

keywords = "Inner mitochondrial membrane, Mitochondrial channel, Patch-clamp, Pharmacology",

author = "Antonenko, {Yuri N.} and Kinnally, {Kathleen W.} and Sean Perini and Henry Tedeschi",

note = "Funding Information: Acknorclledget?rents: WC fould like to thank Carmen Mannella, Charles Bowman and Charles Allen for critical reading of the maNSCriPt. Aided in part by Grant DCB-8818432f rom NSF and Grant IA-AEMP-G 193692fr om USIA.",

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doi = "10.1016/0014-5793(91)80731-H",

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T1 - Selective effect of inhibitors on inner mitochondrial membrane channels

AU - Antonenko, Yuri N.

AU - Kinnally, Kathleen W.

AU - Perini, Sean

AU - Tedeschi, Henry

N1 - Funding Information: Acknorclledget?rents: WC fould like to thank Carmen Mannella, Charles Bowman and Charles Allen for critical reading of the maNSCriPt. Aided in part by Grant DCB-8818432f rom NSF and Grant IA-AEMP-G 193692fr om USIA.

PY - 1991/7/8

Y1 - 1991/7/8

N2 - The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20% (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.

AB - The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20% (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.

KW - Inner mitochondrial membrane

KW - Mitochondrial channel

KW - Patch-clamp

KW - Pharmacology

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SP - 89

EP - 93

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Selective effect of inhibitors on inner mitochondrial membrane channels

Abstract

Keywords

ASJC Scopus subject areas

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