Selective filtration of NMR signals arising from weakly- and strongly-coupled spin systems

Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for analyzing chemical and biological systems. However, in complex solutions with similar molecular components, NMR signals can overlap, making it challenging to distinguish and quantify individual species. In this paper, we introduce new spectral editing sequences that exploit the differences in nuclear spin interactions (J-couplings) between weakly- and strongly-coupled two-spin systems. These sequences selectively attenuate or nullify undesired spin magnetization while they preserve the desired signals, resulting in simplified NMR spectra and potentially facilitating single-species imaging applications. We demonstrate the effectiveness of our approach using a ³¹P spectral filtration method on a model system of nicotinamide dinucleotide (NAD), which exists in oxidized (NAD⁺) and reduced (NADH) forms. The presented sequences are robust to field inhomogeneity, do not require additional sub-spectra, and retain a significant portion of the original signal.