Selective isolation of hyaluronan by solid phase adsorption to silica

Rebecca MacLeod, Fok Vun Chan, Han Yuan, Xin Ye, Yun Jin Ashley Sin, Teraesa M. Vitelli, Tudor Cucu, Annie Leung, Irene Baljak, Samantha Osinski, Yuhong Fu, Gyu Ik Daniel Jung, Anant Amar, Paul L. DeAngelis, Urban Hellman, Mary K. Cowman

Research output: Contribution to journalArticlepeer-review


A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.

Original languageEnglish (US)
Article number114769
JournalAnalytical Biochemistry
StatePublished - Sep 1 2022


  • Conditioned medium
  • Glycosaminoglycan
  • Hyaluronan
  • Isolation
  • Serum
  • Silica

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Selective isolation of hyaluronan by solid phase adsorption to silica'. Together they form a unique fingerprint.

Cite this