Selective isolation of hyaluronan by solid phase adsorption to silica

Rebecca MacLeod; Fok Vun Chan; Han Yuan; Xin Ye; Yun Jin Ashley Sin; Teraesa M. Vitelli; Tudor Cucu; Annie Leung; Irene Baljak; Samantha Osinski; Yuhong Fu; Gyu Ik Daniel Jung; Anant Amar; Paul L. DeAngelis; Urban Hellman; Mary K. Cowman

doi:10.1016/j.ab.2022.114769

Selective isolation of hyaluronan by solid phase adsorption to silica

Rebecca MacLeod, Fok Vun Chan, Han Yuan, Xin Ye, Yun Jin Ashley Sin, Teraesa M. Vitelli, Tudor Cucu, Annie Leung, Irene Baljak, Samantha Osinski, Yuhong Fu, Gyu Ik Daniel Jung, Anant Amar, Paul L. DeAngelis, Urban Hellman, Mary K. Cowman

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.

Original language	English (US)
Article number	114769
Journal	Analytical Biochemistry
Volume	652
DOIs	https://doi.org/10.1016/j.ab.2022.114769
State	Published - Sep 1 2022

Keywords

Conditioned medium
Glycosaminoglycan
Hyaluronan
Isolation
Serum
Silica

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2022.114769

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title = "Selective isolation of hyaluronan by solid phase adsorption to silica",

abstract = "A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.",

keywords = "Conditioned medium, Glycosaminoglycan, Hyaluronan, Isolation, Serum, Silica",

author = "Rebecca MacLeod and Chan, {Fok Vun} and Han Yuan and Xin Ye and Sin, {Yun Jin Ashley} and Vitelli, {Teraesa M.} and Tudor Cucu and Annie Leung and Irene Baljak and Samantha Osinski and Yuhong Fu and Jung, {Gyu Ik Daniel} and Anant Amar and DeAngelis, {Paul L.} and Urban Hellman and Cowman, {Mary K.}",

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doi = "10.1016/j.ab.2022.114769",

language = "English (US)",

volume = "652",

journal = "Analytical Biochemistry",

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T1 - Selective isolation of hyaluronan by solid phase adsorption to silica

AU - MacLeod, Rebecca

AU - Chan, Fok Vun

AU - Yuan, Han

AU - Ye, Xin

AU - Sin, Yun Jin Ashley

AU - Vitelli, Teraesa M.

AU - Cucu, Tudor

AU - Leung, Annie

AU - Baljak, Irene

AU - Osinski, Samantha

AU - Fu, Yuhong

AU - Jung, Gyu Ik Daniel

AU - Amar, Anant

AU - DeAngelis, Paul L.

AU - Hellman, Urban

AU - Cowman, Mary K.

PY - 2022/9/1

Y1 - 2022/9/1

N2 - A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.

AB - A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.

KW - Conditioned medium

KW - Glycosaminoglycan

KW - Hyaluronan

KW - Isolation

KW - Serum

KW - Silica

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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ER -

Selective isolation of hyaluronan by solid phase adsorption to silica

Abstract

Keywords

ASJC Scopus subject areas

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