Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY

G. Esposito; P. Viglino; F. Fogolari; M. Gaestel; J. A. Carver

doi:10.1006/jmre.1998.1430

Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY

G. Esposito, P. Viglino, F. Fogolari, M. Gaestel, J. A. Carver

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Soc. 116, 362-368, 1994) is applied to measure the mobility of the flexible extensions in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental protocol and parameters are discussed in order to employ a simplified data analysis procedure. Further experimental verification of the proposed strategy is also presented using the cyclic peptide gramicidin S as a model compound. Under suitable conditions, the determinations based on the analysis of QUIET-NOESY data are affected to a negligible extent by the approximations that are introduced by the proposed approach.

Original language	English (US)
Pages (from-to)	204-213
Number of pages	10
Journal	Journal of Magnetic Resonance
Volume	132
Issue number	2
DOIs	https://doi.org/10.1006/jmre.1998.1430
State	Published - Jun 1998

Keywords

Correlation-time determination
Cross-relaxation rate determination
Hsp25
Local mobility
QUIET-NOESY
Selective NOE
Small heat-shock proteins

ASJC Scopus subject areas

Biophysics
Biochemistry
Nuclear and High Energy Physics
Condensed Matter Physics

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10.1006/jmre.1998.1430

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@article{159636040d7944d290921f2a91a3ef91,

title = "Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY",

abstract = "The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Soc. 116, 362-368, 1994) is applied to measure the mobility of the flexible extensions in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental protocol and parameters are discussed in order to employ a simplified data analysis procedure. Further experimental verification of the proposed strategy is also presented using the cyclic peptide gramicidin S as a model compound. Under suitable conditions, the determinations based on the analysis of QUIET-NOESY data are affected to a negligible extent by the approximations that are introduced by the proposed approach.",

keywords = "Correlation-time determination, Cross-relaxation rate determination, Hsp25, Local mobility, QUIET-NOESY, Selective NOE, Small heat-shock proteins",

author = "G. Esposito and P. Viglino and F. Fogolari and M. Gaestel and Carver, {J. A.}",

note = "Funding Information: This work was supported by grants from the Italian Government to JAC and from the CNR (Consiglio Nazionale delle Ricerche) to GE. The authors thank Prof. G. Bodenhausen for reading the manuscript. The comments of Dr. A. Makek are also acknowledged.",

year = "1998",

month = jun,

doi = "10.1006/jmre.1998.1430",

language = "English (US)",

volume = "132",

pages = "204--213",

journal = "Journal of Magnetic Resonance",

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publisher = "Academic Press Inc.",

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T2 - Implementation and Analysis of QUIET-NOESY

AU - Esposito, G.

AU - Viglino, P.

AU - Fogolari, F.

AU - Gaestel, M.

AU - Carver, J. A.

N1 - Funding Information: This work was supported by grants from the Italian Government to JAC and from the CNR (Consiglio Nazionale delle Ricerche) to GE. The authors thank Prof. G. Bodenhausen for reading the manuscript. The comments of Dr. A. Makek are also acknowledged.

PY - 1998/6

Y1 - 1998/6

N2 - The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Soc. 116, 362-368, 1994) is applied to measure the mobility of the flexible extensions in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental protocol and parameters are discussed in order to employ a simplified data analysis procedure. Further experimental verification of the proposed strategy is also presented using the cyclic peptide gramicidin S as a model compound. Under suitable conditions, the determinations based on the analysis of QUIET-NOESY data are affected to a negligible extent by the approximations that are introduced by the proposed approach.

AB - The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Soc. 116, 362-368, 1994) is applied to measure the mobility of the flexible extensions in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental protocol and parameters are discussed in order to employ a simplified data analysis procedure. Further experimental verification of the proposed strategy is also presented using the cyclic peptide gramicidin S as a model compound. Under suitable conditions, the determinations based on the analysis of QUIET-NOESY data are affected to a negligible extent by the approximations that are introduced by the proposed approach.

KW - Correlation-time determination

KW - Cross-relaxation rate determination

KW - Hsp25

KW - Local mobility

KW - QUIET-NOESY

KW - Selective NOE

KW - Small heat-shock proteins

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IS - 2

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Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY

Abstract

Keywords

ASJC Scopus subject areas

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