Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY

G. Esposito, P. Viglino, F. Fogolari, M. Gaestel, J. A. Carver

Research output: Contribution to journalArticlepeer-review


The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Soc. 116, 362-368, 1994) is applied to measure the mobility of the flexible extensions in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental protocol and parameters are discussed in order to employ a simplified data analysis procedure. Further experimental verification of the proposed strategy is also presented using the cyclic peptide gramicidin S as a model compound. Under suitable conditions, the determinations based on the analysis of QUIET-NOESY data are affected to a negligible extent by the approximations that are introduced by the proposed approach.

Original languageEnglish (US)
Pages (from-to)204-213
Number of pages10
JournalJournal of Magnetic Resonance
Issue number2
StatePublished - Jun 1998


  • Correlation-time determination
  • Cross-relaxation rate determination
  • Hsp25
  • Local mobility
  • Selective NOE
  • Small heat-shock proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics


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