Self-assembled DNA crystals: The impact on resolution of 5′-phosphates and the DNA source

Ruojie Sha, Jens J. Birktoft, Nam Nguyen, Arun Richard Chandrasekaran, Jianping Zheng, Xinshuai Zhao, Chengde Mao, Nadrian C. Seeman

Research output: Contribution to journalArticle

Abstract

Designed self-assembled DNA crystals consist of rigid DNA motifs that are held together by cohesive sticky-ended interactions. A prominent application of such systems is that they might be able to act as macromolecular hosts for macromolecular guests, thereby alleviating the crystallization problem of structural biology. We have recently demonstrated that it is indeed possible to design and construct such crystals and to determine their structures by X-ray diffraction procedures. To act as useful hosts that organize biological macromolecules for crystallographic purposes, maximizing the resolution of the crystals will maximize the utility of the approach. The structures reported so far have diffracted only to about 4 Å, so we have examined two factors that might have impact on the resolution. We find no difference in the resolution whether the DNA is synthetic or PCR-generated. However, we find that the presence of a phosphate on the 5′-end of the strands improves the resolution of the crystals markedly.

Original languageEnglish (US)
Pages (from-to)793-797
Number of pages5
JournalNano Letters
Volume13
Issue number2
DOIs
StatePublished - Feb 13 2013

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Keywords

  • Self-assembled 3D DNA crystals
  • X-ray diffraction
  • crystal resolution
  • designed crystals
  • natural and synthetic DNA
  • phosphorylated DNA

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Sha, R., Birktoft, J. J., Nguyen, N., Chandrasekaran, A. R., Zheng, J., Zhao, X., Mao, C., & Seeman, N. C. (2013). Self-assembled DNA crystals: The impact on resolution of 5′-phosphates and the DNA source. Nano Letters, 13(2), 793-797. https://doi.org/10.1021/nl304550c