TY - JOUR
T1 - Self-assembly of stimuli-responsive coiled-coil fibrous hydrogels
AU - Meleties, Michael
AU - Katyal, Priya
AU - Lin, Bonnie
AU - Britton, Dustin
AU - Montclare, Jin Kim
N1 - Funding Information:
The authors would like to acknowledge NYU Langone’s Microscopy Laboratory. This shared resource is partially supported by the Cancer Center Support Grant P30CA016087 at the Laura and Isaac Perlmutter Cancer Center. We also thank Dr Jeffrey F. Morris and Nelya Akhmetkhanova for providing access to the rheometer at The City College of New York.
Funding Information:
This work was supported by NSF-DMREF under Award Number DMR 1728858, and NSF-MRSEC Program under Award Number DMR 1420073.
Funding Information:
The authors would like to acknowledge NYU Langone's Microscopy Laboratory. This shared resource is partially supported by the Cancer Center Support Grant P30CA016087 at the Laura and Isaac Perlmutter Cancer Center. We also thank Dr Jeffrey F. Morris and Nelya Akhmetkhanova for providing access to the rheometer at The City College of New York.
Publisher Copyright:
© The Royal Society of Chemistry 2021.
PY - 2021/7/14
Y1 - 2021/7/14
N2 - Owing to their tunable properties, hydrogels comprised of stimuli-sensitive polymers are one of the most appealing scaffolds with applications in tissue engineering, drug delivery and other biomedical fields. We previously reported a thermoresponsive hydrogel formed using a coiled-coil protein, Q. Here, we expand our studies to identify the gelation of Q protein at distinct pH conditions, creating a protein hydrogel system that is sensitive to temperature and pH. Through secondary structure analysis, transmission electron microscopy, and rheology, we observed that Q self-assembles and forms fiber-based hydrogels exhibiting upper critical solution temperature behavior with increased elastic properties at pH 7.4 and pH 10. At pH 6, however, Q forms polydisperse nanoparticles, which do not further self-assemble and undergo gelation. The high net positive charge of Q at pH 6 creates significant electrostatic repulsion, preventing its gelation. This study will potentially guide the development of novel scaffolds and functional biomaterials that are sensitive towards biologically relevant stimuli.
AB - Owing to their tunable properties, hydrogels comprised of stimuli-sensitive polymers are one of the most appealing scaffolds with applications in tissue engineering, drug delivery and other biomedical fields. We previously reported a thermoresponsive hydrogel formed using a coiled-coil protein, Q. Here, we expand our studies to identify the gelation of Q protein at distinct pH conditions, creating a protein hydrogel system that is sensitive to temperature and pH. Through secondary structure analysis, transmission electron microscopy, and rheology, we observed that Q self-assembles and forms fiber-based hydrogels exhibiting upper critical solution temperature behavior with increased elastic properties at pH 7.4 and pH 10. At pH 6, however, Q forms polydisperse nanoparticles, which do not further self-assemble and undergo gelation. The high net positive charge of Q at pH 6 creates significant electrostatic repulsion, preventing its gelation. This study will potentially guide the development of novel scaffolds and functional biomaterials that are sensitive towards biologically relevant stimuli.
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U2 - 10.1039/d1sm00780g
DO - 10.1039/d1sm00780g
M3 - Article
C2 - 34137426
AN - SCOPUS:85109210395
VL - 17
SP - 6470
EP - 6476
JO - Soft Matter
JF - Soft Matter
SN - 1744-683X
IS - 26
ER -