TY - JOUR
T1 - Self-association of hyaluronate segments in aqueous NaCl solution
AU - Turner, Raymond E.
AU - Lin, Pinyen
AU - Cowman, Mary K.
N1 - Funding Information:
cussions. This research was supported by National Institutes of Health Grant EY 04804. Preliminary accounts of this work were presented at the 31st Annual Meeting of the Biophysical Society, February 22-26, 1987, New Orleans, Louisiana (39), and at the Annual Meeting of the Association for Research in and Ophthalmology, May 4-8, 1987, Sarasota, (40).
PY - 1988/9
Y1 - 1988/9
N2 - The potential for self-association by hyaluronate (HA) chains in 0.15 m NaCl was investigated, using low molecular weight HA segments as a model system. HA segments were derived from the polymer by controlled enzymatic digestion, and purified by gel filtration chromatography. Seven samples of narrow molecular weight distribution were analyzed by sensitivity-enhanced polyacrylamide gel electrophoresis, and found to have the following weight-average numbers of repeating disaccharide units: A, 90; B, 51; C, 38; D, 31; E, 23; F, 18; G, 13. The segment preparations were studied in 0.15 m NaCl by capillary viscometry, low angle laser light scattering, and circular dichroism spectroscopy. The data indicate concentration-dependent intermolecular association of short segments, and a capability for intramolecular association (hairpin formation) by larger HA segments.
AB - The potential for self-association by hyaluronate (HA) chains in 0.15 m NaCl was investigated, using low molecular weight HA segments as a model system. HA segments were derived from the polymer by controlled enzymatic digestion, and purified by gel filtration chromatography. Seven samples of narrow molecular weight distribution were analyzed by sensitivity-enhanced polyacrylamide gel electrophoresis, and found to have the following weight-average numbers of repeating disaccharide units: A, 90; B, 51; C, 38; D, 31; E, 23; F, 18; G, 13. The segment preparations were studied in 0.15 m NaCl by capillary viscometry, low angle laser light scattering, and circular dichroism spectroscopy. The data indicate concentration-dependent intermolecular association of short segments, and a capability for intramolecular association (hairpin formation) by larger HA segments.
UR - http://www.scopus.com/inward/record.url?scp=0023759939&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023759939&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(88)90153-1
DO - 10.1016/0003-9861(88)90153-1
M3 - Article
C2 - 3421721
AN - SCOPUS:0023759939
SN - 0003-9861
VL - 265
SP - 484
EP - 495
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -