Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1.

D. G. Albertson; R. Fishpool; P. Sherrington; E. Nacheva; C. Milstein

doi:10.1002/j.1460-2075.1988.tb03135.x

Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1.

D. G. Albertson, R. Fishpool, P. Sherrington, E. Nacheva, C. Milstein

Oral and Maxillofacial Surgery

Research output: Contribution to journal › Article › peer-review

Abstract

A method for in situ hybridization originally developed for mapping genes in the nematode, Caenorhabditis elegans has been adapted for high resolution cytological mapping of genes in the human. The probe DNAs are labelled by incorporation of biotin dUTP and the site of hybridization detected by immunofluorescence. For the accurate assignment of the hybridization signal to chromosome bands, visualized by staining with Hoechst 33258, a heterologous ribosomal DNA probe is also included in the hybridization reaction. These rDNA signals are used as fiducial markers when aligning the two fluorescent images. We demonstrate the method by assignment of the human thymocyte CD1 antigen genes to human chromosome 1q22-23.

Original language	English (US)
Pages (from-to)	2801-2805
Number of pages	5
Journal	The EMBO journal
Volume	7
Issue number	9
DOIs	https://doi.org/10.1002/j.1460-2075.1988.tb03135.x
State	Published - 1988

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

Access to Document

10.1002/j.1460-2075.1988.tb03135.x

Cite this

@article{b12dcdaf78374c75beee95e84725ff66,

title = "Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1.",

abstract = "A method for in situ hybridization originally developed for mapping genes in the nematode, Caenorhabditis elegans has been adapted for high resolution cytological mapping of genes in the human. The probe DNAs are labelled by incorporation of biotin dUTP and the site of hybridization detected by immunofluorescence. For the accurate assignment of the hybridization signal to chromosome bands, visualized by staining with Hoechst 33258, a heterologous ribosomal DNA probe is also included in the hybridization reaction. These rDNA signals are used as fiducial markers when aligning the two fluorescent images. We demonstrate the method by assignment of the human thymocyte CD1 antigen genes to human chromosome 1q22-23.",

author = "Albertson, {D. G.} and R. Fishpool and P. Sherrington and E. Nacheva and C. Milstein",

year = "1988",

doi = "10.1002/j.1460-2075.1988.tb03135.x",

language = "English (US)",

volume = "7",

pages = "2801--2805",

journal = "The EMBO journal",

issn = "0261-4189",

publisher = "EMBO Press",

number = "9",

}

TY - JOUR

T1 - Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes

T2 - assignment of the human thymocyte CD1 antigen genes to chromosome 1.

AU - Albertson, D. G.

AU - Fishpool, R.

AU - Sherrington, P.

AU - Nacheva, E.

AU - Milstein, C.

PY - 1988

Y1 - 1988

N2 - A method for in situ hybridization originally developed for mapping genes in the nematode, Caenorhabditis elegans has been adapted for high resolution cytological mapping of genes in the human. The probe DNAs are labelled by incorporation of biotin dUTP and the site of hybridization detected by immunofluorescence. For the accurate assignment of the hybridization signal to chromosome bands, visualized by staining with Hoechst 33258, a heterologous ribosomal DNA probe is also included in the hybridization reaction. These rDNA signals are used as fiducial markers when aligning the two fluorescent images. We demonstrate the method by assignment of the human thymocyte CD1 antigen genes to human chromosome 1q22-23.

AB - A method for in situ hybridization originally developed for mapping genes in the nematode, Caenorhabditis elegans has been adapted for high resolution cytological mapping of genes in the human. The probe DNAs are labelled by incorporation of biotin dUTP and the site of hybridization detected by immunofluorescence. For the accurate assignment of the hybridization signal to chromosome bands, visualized by staining with Hoechst 33258, a heterologous ribosomal DNA probe is also included in the hybridization reaction. These rDNA signals are used as fiducial markers when aligning the two fluorescent images. We demonstrate the method by assignment of the human thymocyte CD1 antigen genes to human chromosome 1q22-23.

UR - http://www.scopus.com/inward/record.url?scp=0024076254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024076254&partnerID=8YFLogxK

U2 - 10.1002/j.1460-2075.1988.tb03135.x

DO - 10.1002/j.1460-2075.1988.tb03135.x

M3 - Article

C2 - 3053166

AN - SCOPUS:0024076254

SN - 0261-4189

VL - 7

SP - 2801

EP - 2805

JO - The EMBO journal

JF - The EMBO journal

IS - 9

ER -

Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1.

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this