Sensitive, label-free DNA diagnostics based on near-field microwave imaging

Barry Friedman, Mariafrancis A. Gaspar, Sergey Kalachikov, Kiejin Lee, Rastislav Levicky, Gang Shen, Hyunjun Yoo

Research output: Contribution to journalArticle

Abstract

Biological assays often rely on "reporter labels" to enhance measurement sensitivity, for example, by incorporation of a fluorescent dye or a nanoparticle into a nucleic acid or a protein. Use of labels, however, complicates sample preparation, increases assay costs, and can cause experimental artifacts by interfering with assay thermodynamics or limiting label stability. We evaluate near-field microwave imaging (NFMI) as an alternative, label-free technique for molecular diagnostics. Using DNA monolayers as an experimental model, NFMI is demonstrated to achieve sensitivities comparable to conventional fluorescence bioassays. Moreover, NFMI is shown to be compatible with imaging at resolutions required by microarray applications, as demonstrated by monitoring DNA hybridization in an array format.

Original languageEnglish (US)
Pages (from-to)9666-9667
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number27
DOIs
StatePublished - Jul 13 2005

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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