Separation and Properties of Rabbit Buccal Mucosal Wound Hyaluronidase

C. N. Bertolami, R. H. Day, D. G. Ellis

Research output: Contribution to journalArticle

Abstract

This study establishes the existence of a mammalian buccal mucosal wound hyaluronidase (hyaluronate 4-glycohydrolase; EC 3.2.1.35) having properties distinct from those of the endogenous lysosomal hyaluronidase of normal (uninjured) buccal mucosa. A time-dependent change in hyaluronidase activity was measured, with the highest specific activity occurring on post-wound day 4 (7.7 ± 1.3 unitslmg protein), followed by consecutive decreases until activity was no longer discernible by day 21. Mucosal wound hyaluronidase closely resembled a previously described integumentary wound endoglycosidase in terms of a high pH optimum (5.0-6.0), distinct (but non-exclusive) substrate preference for hyaluronic acid, and ability to generate saturated depolymerization products by an endoglycosidic hydrolysis.

Original languageEnglish (US)
Pages (from-to)939-944
Number of pages6
JournalJournal of dental research
Volume65
Issue number6
DOIs
StatePublished - Jun 1986

ASJC Scopus subject areas

  • Dentistry(all)

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